摘要:
为研究重金属离子对褶纹冠Cu/ZnSOD基因mRNA表达和酶活性的影响,采用Cd2+,Cu2+和Pb2+单一胁迫褶纹冠蚌72h,检测0,6,12,24,48和72 h,Cu/ZnSODmRNA表达和酶活性的变化.结果表明:在Cd2+胁迫下,Cu/ZnSODmRNA表达量及酶活性均随暴露时间延长而逐渐升高,72h达到峰值,且48和72 h均显著性高于对照组(P<0.05);在Cu2+胁迫下,Cu/ZnSOD mRNA表达量及酶活性具有先升高后降低的趋势,12 h两者达到峰值,其中,Cu/ZnSOD mRNA表达量在12 h显著高于对照组(P<0.05),Cu/ZnSOD酶活性在6,24,48和72 h的表达量均显著高于对照组(P<0.05);Pb2+胁迫与Cu2+胁迫趋势相似,Cu/ZnSODmRNA表达量及酶活性均在48 h达到峰值,Cu/ZnSODmRNA表达量在24,48和72 h显著性高于对照组(P<0.05),Cu/ZnSOD酶活性在48和72 h的表达量均显著高于对照组(P<0.05).说明褶纹冠蚌内脏团中Cu/ZnSODmRNA和酶可以作为生物标志物对水环境中的重金属污染进行检测.%To investigate the effects of heavy metal ions on the mRNA expression and enzyme activity of Cu/ZnSOD gene in Cristaria plicata,the Cristaria plicata was stressed for 72h using heavy metal ions (Cu2+,Cd2+ and Pb2+),detecting the changes of mRNA expression level and enzyme activities of Cu/Zn-SOD gene at different time points.The results showed that under Cd2+ stress,the mRNA expression level and enzyme activity of Cu/ZnSOD were gradually increased with exposure time and reached peak at 72h, and they were significantly higher in the experimental group than those in the control group(P<0.05)at 48 and 72 h.Under Cu2+ stress,the mRNA expression level and enzyme activity of Cu/ZnSOD firstly in-creased with time until peaking at 12 h (P<0.05),then decreased gradually.Moreover,the mRNA expres-sion level of Cu/Zn SOD at 12 h and the enzyme activity of Cu/Zn SOD at 6,24,48 and 72 h in the experi-mental group were significantly higher than those in the control group(P<0.05).Under Pb2+ stress,the trends of mRNA expression level and enzyme activities of Cu/ZnSOD were similar to those under Cu2+stress with both peaking at 48 h.Compared to the control group,the mRNA expression level of Cu/ZnSOD at 24,48 and 72 h and the enzyme activities of Cu/Zn SOD at 48 and 72 h were significantly higher in the experimental group(P<0.05).The results showed that the change of mRNA expression level and enzyme activity of Cu/ZnSOD gene in gill tissue of Cristaria plicata could be used as biomarkers to detect and eval-uate the heavy metal pollution in aquatic environment.