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首页> 外文会议>13th International Symposium on Ceramics in Medicine and the Annual Meeting of the International Society for Ceramics in Medicine 22-26 November 2000 Bologna, Italy >Analysis of Osteoblast Activity around Beta-TCP Particles Implanted into Bone: Expression of Bone Matrix Protein mRNAs
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Analysis of Osteoblast Activity around Beta-TCP Particles Implanted into Bone: Expression of Bone Matrix Protein mRNAs

机译：植入骨的β-TCP颗粒周围成骨细胞活性的分析：骨基质蛋白mRNA的表达。

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摘要

Osteoblast activity around beta-tricalcium phosphate (beta-TCP) particles (150-300 mum in diameter) implanted into rat tibiae was analyzed by in situ hybridization with digoxyenin-labeled procollagen alpha1(I) (COL), osteonectin (ON), osteocalcin (OC) and osteopontin (OPN) RNA probes. Holes without implantation were used as a control. Specimens were collected at 3, 5, 7 and 10 days after the operation. New bond was formed centripetally in both the beta-TCP implanted and control groups, and all four kinds of mRNA were expressed in activated osteoblasts. A COL signal was expressed most strongly and widely, and was detected at the peripheral region of the hole at day 3. The other three mRNAs were also expressed in bone forming osteoblasts by day 7. However, in the earlier cell reaction stage, OPN expression in the beta-TCP implanted group was different from that in the control group: OPN mRNA was seen exclusively in the cell on the particles, and an OPN signal was detected not only in COL-positive cells, but also in COL-negative cells. The former cells may be osteoblasts and reflect the early process of bone formation on biomaterials. The latter cells may be macrophages and reflect foreign body reactions. Expression of these OPN mRNAs induced by implantation of beta-TCP may play a role in bone formation on the materials and in determining the their biocompatibility.
机译：通过与地高辛素标记的胶原蛋白原胶原α1（I）（COL），骨连接素（ON），骨钙素原位杂交，分析了植入到胫骨中的β-磷酸三钙（β-TCP）颗粒（直径为150-300微米）周围的成骨细胞活性（OC）和骨桥蛋白（OPN）RNA探针。未植入的孔用作对照。手术后第3、5、7和10天收集标本。在植入β-TCP的对照组和对照组中均向心形成新的键，并且所有四种mRNA在活化的成骨细胞中表达。在第3天，COL信号表达最强，最广泛，并在孔的外围区域检测到。在第7天，其他三个mRNA在成骨成骨细胞中也表达。但是，在较早的细胞反应阶段，OPN表达β-TCP植入组中的OPN与对照组不同：仅在颗粒上的细胞中观察到OPN mRNA，不仅在COL阳性细胞中而且在COL阴性细胞中都检测到OPN信号。前一种细胞可能是成骨细胞，反映了生物材料上骨形成的早期过程。后者的细胞可能是巨噬细胞并反映异物反应。通过植入β-TCP诱导的这些OPN mRNA的表达可能在材料上的骨形成以及确定其生物相容性中起作用。

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