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Preparation of Amino-silane Modified Superparamagnetic Fe3O4 Nanoparticles and Application for Lipase Immobilization

机译:氨基硅烷修饰的超顺磁性Fe3O4纳米粒子的制备及其在脂肪酶固定化中的应用

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1.Introduction Magnetic microspheres have been showing great potential applications in biomedical and bioengineering because of their large surface area and facile magnetic separation[1-3].These magnetic microspheres can be covalently connected with enzymes. However, the interactions between enzymes and magnetic supports are not quite thoroughly. In this work, tetraethyl orthosilicate (TEOS) were employed to introduce highly reactive silanols on the surface of Fe3O4. Then they were modified with γ-aminopropyltriethoxysilane(APTS). The Fe3O4/SiOx-APTS were used to lipase immobilization with two strategies.
机译:1.引言磁性微球由于具有较大的表面积和易分离的磁性而在生物医学和生物工程领域具有巨大的应用前景[1-3]。这些磁性微球可以与酶共价连接。但是,酶与磁性载体之间的相互作用还不够彻底。在这项工作中,使用原硅酸四乙酯(TEOS)在Fe3O4表面引入高反应性硅烷醇。然后用γ-氨基丙基三乙氧基硅烷(APTS)对其进行改性。 Fe3O4 / SiOx-APTS用于脂肪酶的固定化有两种策略。

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