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首页> 外文会议>2014 40th Annual Northeast Bioengineering Conference >Continuous particle sorting using three dimensional insulator based dielectrophoresis
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Continuous particle sorting using three dimensional insulator based dielectrophoresis

机译：使用基于三维绝缘子的介电泳进行连续粒子分选

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Low frequency insulator based dielectrophoresis (iDEP) is a promising technique to study cell surface dielectric properties. To date, iDEP has been exploited to distinguish, characterize, and manipulate particles and bacteria based on their size and general cell phenotype (e.g. gram positive vs. gram negative). However, separation of bacteria with diverse surface phenotypes but similar sizes sets a much higher demand on separation sensitivity, necessitating improvement in channel structure design in order to increase the electrical field gradient. In this work, a three dimensional insulator based dielectrophoresis (3DiDEP) microdevice is designed to achieve continuous particle sorting based on their size and, more importantly, on their surface dielectric properties. A 3D constriction is fabricated inside Poly (methyl methacrylate) (PMMA) channels using a micromilling technique. By controlling the channel geometry at the 3D constriction area, a nonuniform electric field with a large intensity gradient perpendicular to the local particle flow direction results in transverse particle deflection, driving particles into different outlet streams. With both simulation and experiments, we will show that a diverse array of particles and cells can be distinguished by 3DiDEP. This 3DiDEP sorter can be used in multiple applications in which the surface properties of cells or particles are of special interest.
机译：基于低频绝缘体的介电电泳（iDEP）是研究细胞表面介电特性的有前途的技术。迄今为止，iDEP已被用来根据颗粒和细菌的大小和一般细胞表型（例如，革兰氏阳性与革兰氏阴性）来区分，表征和操作颗粒和细菌。但是，分离具有不同表面表型但大小相似的细菌对分离灵敏度提出了更高的要求，因此有必要改进通道结构设计以增加电场梯度。在这项工作中，基于三维绝缘子的介电电泳（3DiDEP）微器件被设计为基于其尺寸，更重要的是基于其表面介电特性实现连续的颗粒分类。使用微铣削技术在聚（甲基丙烯酸甲酯）（PMMA）通道内部制造了3D收缩部。通过控制3D收缩区域的通道几何形状，垂直于局部粒子流向的具有大强度梯度的不均匀电场会导致横向粒子偏转，从而将粒子驱动到不同的出口流中。通过仿真和实验，我们将显示3DiDEP可以区分出各种各样的粒子和细胞。该3DiDEP分选器可用于细胞或颗粒表面特性特别受关注的多种应用中。

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