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首页> 外文会议>第四届国际板栗大会（Proceedings of Fourth International Chestnut Symposium) >Shoot Apex Culture of Dormant Buds in Chestnut
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Shoot Apex Culture of Dormant Buds in Chestnut

机译：拍摄栗子休眠芽的尖顶培养

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Chestnuts (Castanea spp.) can be propagated by tissue culture methods such as meristem tip culture, shoot tip culture and bud culture. However, some limiting factors prevent using these methods extensively. For example, the explant collection periods of these methods are short, and the collection time is critical. In addition, shoot formation ratios of explants on the initial media and survival of these shootlets thereafter are generally low due to factors such as infection, browning, and vitrification. In this study the shoot apex of the dormant buds were used as the explants source for the first time. By use of this method the explant collection period was much longer and the mass multiplication probability increased. Three chestnut cultivars native to Turkey -'Sarraslama', 'Osmanoglu' and 'Haclomer' Castanea sativa Mill. -were used in the study in two years of experiments 2007 and 2008. Two main media were used as initial to obtain shootlets: MS (I/2×NH4NO3 and KNO3) and DKW (Driver-Kuniyuki walnut medium). In multiplication step MS (1/2×NH4NO3 medium was used with 0.5, 1.0, 1.5 mg/L BAP concentrations and in rooting MS (I/2×NH4NO3 with 1.0 and 2.0 mg/L IBA concentrations were used. Shootlet formation from the explants in 2008 was higher (66.6695.45%) than in 2007 (42.00-75.00%). MS (1/2×NH4NO3 and KNO3)medium has given successful results in this respect. Therefore it can be recommended as a growing medium. The multiplication coefficient varied between 3.12 to 3.57 in 2007 and 3.95 to 4.76 in 2008 according to the cuitivars. Rooting of the shootlets was difficult, and no rooted shootlet was obtained. However the experiment is now being continued, and new rooting media will be tested. The results obtained from this study showed that this method can be efficiently used in the mass multiplication of chestnuts.
机译：栗子（Castanea spp。）可以通过分生组织尖端培养，枝梢培养和芽培养等组织培养方法繁殖。但是，一些限制因素阻止了这些方法的广泛使用。例如，这些方法的外植体收集期短，并且收集时间很关键。另外，由于诸如感染，褐变和玻璃化的因素，外植体在初始培养基上的芽形成比例和这些芽的存活率通常较低。在这项研究中，休眠芽的茎尖首次被用作外植体来源。通过使用这种方法，外植体的收集期更长，并且大量繁殖的可能性增加。土生土长的三种栗树品种-'Sarraslama'，'Osmanoglu'和'Haclomer'Castanea sativa Mill。 -分别在2007年和2008年的两年实验中用于该研究。最初使用两种主要培养基来获得子弹：MS（I / 2×NH4NO3和KNO3）和DKW（Driver-Kuniyuki胡桃培养基）。在增殖步骤中，MS（1/2×NH4NO3培养基的BAP浓度为0.5、1.0、1.5 mg / L，生根MS（I / 2×NH4NO3的IBA浓度为1.0和2.0 mg / L）。 2008年的外植体含量（66.6695.45％）高于2007年的（42.00-75.00％），MS（1/2×NH4NO3和KNO3）培养基在这方面取得了成功的结果，因此可以推荐作为生长培养基。根据瓜类，倍增系数在2007年的3.12至3.57和2008年的3.95至4.76之间变化，子粒的生根很困难，没有生根的子粒，但是实验仍在继续，并且将测试新的生根培养基从这项研究中获得的结果表明，该方法可以有效地用于栗子的大量繁殖。

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