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首页> 外文会议>第四届国际板栗大会（Proceedings of Fourth International Chestnut Symposium) >Biodiversity of Chestnut Germplasm (Castanea sativa Mill.) Grown in North-West Italy
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Biodiversity of Chestnut Germplasm (Castanea sativa Mill.) Grown in North-West Italy

机译：意大利西北部种植的板栗种质（Castanea sativa Mill。）的生物多样性

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摘要

Chestnut (Castanea sativa Mill.) is a multipurpose species widely spread in Europe. Italy is the main European producer and Piedmont (North-West Italy) hosts a reach chestnut germplasm, including minor, often endangered, varieties. Within the EU project MANCHEST most of this germplasm was evaluated and described by DNA, chemical and sensory analyses. A current project funded by the Piedmont Region administration is aimed at using and developing statistical tools, such as PCO, discriminant analysis and neural networks, able to classify and characterize the chestnut biodiversity. DNA from 88 chestnut individual was amplified at 10 SSR loci and then analysed by ABI-Prism 377 sequencer using the Genescan system (Applied Biosystems). Chemical and sensory analyses were performed on a selected number of cultivars chosen on the basis of the results of DNA analysis that identified 42 genotypes. The chemical analysis indicated starch and sugars as the most abundant compounds, followed by total dietary fiber; fat and protein were present in lower amount. Sensory analysis allowed the selection of a group of cultivars and clones particularly appreciated for their taste and aroma. The present work confirmed the aptitude of the cultivars for different uses and underlined the importance of traits such as size, ease of skin removal and absence of epysperm intrusions on product acceptance. Although environmental factors were a significant cause of variability, the use of appropriate statistical methods could greatly enhance the discriminative capacity of studied variables.
机译：板栗（Castanea sativa Mill。）是一种在欧洲广泛传播的多用途物种。意大利是欧洲的主要生产国，皮埃蒙特（意大利西北部）拥有可食用的板栗种质，包括次要品种（通常是濒临灭绝的品种）。在欧盟MANCHEST项目中，大多数种质已通过DNA，化学和感官分析进行了评估和描述。由皮埃蒙特地区政府资助的当前项目旨在使用和开发统计工具，例如PCO，判别分析和神经网络，能够对板栗的生物多样性进行分类和表征。在10个SSR位点扩增来自88个栗子个体的DNA，然后使用Genescan系统（Applied Biosystems）通过ABI-Prism 377测序仪进行分析。根据DNA鉴定的结果，对42个基因型进行了化学和感官分析。化学分析表明，淀粉和糖是最丰富的化合物，其次是膳食纤维。脂肪和蛋白质的含量较低。感官分析允许选择一组因其味道和香气而特别受赞赏的品种和克隆。目前的工作证实了该品种在不同用途上的适应性，并强调了诸如大小，易于脱皮和在产品接受方面没有胚芽侵入等特性的重要性。尽管环境因素是造成变化的重要原因，但使用适当的统计方法可以大大增强研究变量的判别能力。

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