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In-vitro hyperthermia studied by cellular impedance sensors

机译:细胞阻抗传感器研究体外热疗

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In this paper, we used an experimental platform based on cellular impedance sensors to study cellular changes after hyperthermia treatments. Impedance profiling showed high correlation with traditional MTT results, thus the impedance data represent cell viabilities very well. Then, status of cells under six typical hyperthermia treatments with different temperatures between 41???46 ??C and different treating durations (0???30 min, 45 ??C) were examined by the platform separately. The results showed increasing cell damage occurred when temperature or duration increased, and indicated the thresholds of temperature (???43 ??C). Moreover, it is possible that a thermal isoeffect dose (TID) threshold exists in the two cell lines (equivalent time at 43 ??C EM43=30???40 min). These results fit previous theories of hyperthermia, demonstrating that the cellular impedance sensor is a useful analytical tool for the in vitro research of thermal therapy.
机译:在本文中,我们使用了基于细胞阻抗传感器的实验平台来研究热疗后的细胞变化。阻抗谱显示与传统MTT结果高度相关,因此阻抗数据很好地代表了细胞活力。然后,通过平台分别检查在41°C±46°C之间的不同温度和不同处理时间(0°C 30min,45°C)下的六种典型的高温治疗下的细胞状态。结果表明,当温度或持续时间增加时,发生的细胞损伤增加,并指示出温度阈值(≥43℃)。而且,有可能在两个细胞系中都存在热等效效应剂量(TID)阈值(在43°C EM43时的等效时间= 30≤40分钟)。这些结果符合以前的高温理论,表明细胞阻抗传感器是热疗法体外研究的有用分析工具。

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