首页> 外文会议>Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV >Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy using a targeted imaging probe
【24h】

Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy using a targeted imaging probe

机译:使用靶向成像探头检测早期食管恶性肿瘤的近红外荧光内窥镜设计与验证

获取原文
获取原文并翻译 | 示例

摘要

Barrett's esophagus is a condition that predisposes patients to esophageal cancer. Early detection of cancer in these patients can be curative, but is confounded by a lack of contrast in white light endoscopy (WLE). Application of fluorescently-labeled lectins to the esophagus during endoscopy can more accurately delineate dysplasia emerging within Barrett's than WLE, but strong tissue autofluorescence has limited sensitivity and dynamic range of this approach. To overcome this challenge, we synthesized a near-infrared (NIR) fluorescent lectin and have constructed a clinically translatable endoscope for simultaneous WLE and NIR imaging. An imaging fiber bundle, shielded from patient contact using a disposable catheter, relays collected light into an optical path that splits the WL reflectance and NIR emission onto two cameras for simultaneous video-rate recording. The captured images are co-registered and the honeycomb artifact arising from the fiber bundle is removed using interpolation between image points derived from individual fibers. A minimum detectable concentration of 110 nM was determined using a dilution series of IRDye800CW-lectin in black well plates. We have demonstrated the ability to use our endoscope to distinguish between different tissue types in ex vivo mouse stomachs. Future work using human ex vivo tissue specimens will determine safe illumination limits and sensitivity for dysplasia and adenocarcinoma in Barrett's esophagus, prior to commencing clinical trials.
机译:巴雷特食管是一种使患者容易患食道癌的疾病。在这些患者中及早发现癌症是可以治愈的,但是由于白光内窥镜检查(WLE)缺乏对比而感到困惑。与WLE相比,在内窥镜检查期间将荧光标记的凝集素应用于食道可以更准确地描述Barrett内出现的异型增生,但是强大的组织自发荧光限制了该方法的敏感性和动态范围。为了克服这一挑战,我们合成了近红外(NIR)荧光凝集素,并构建了可同时进行WLE和NIR成像的临床可翻译内窥镜。成像光纤束通过使用一次性导管与患者接触而屏蔽,将收集的光中继到一条光路中,该光路将WL反射率和NIR发射分到两个摄像头上,以便同时记录视频速率。对捕获的图像进行配准,并使用从各个纤维派生的图像点之间的插值来消除由纤维束产生的蜂窝伪影。使用黑色孔板中的IRDye800CW-凝集素稀释系列确定最低可检测浓度为110 nM。我们已经证明了使用内窥镜区分离体小鼠胃中不同组织类型的能力。在开始临床试验之前,未来使用人体离体组织标本的工作将确定安全的照明极限和对Barrett食管中异型增生和腺癌的敏感性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号