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Short cationic host defense peptides: determination of sequence requirements for killing Pseudomonas aeruginosa

机译：短阳离子宿主防御肽：确定杀死铜绿假单胞菌的序列要求

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Little is known about sequence requirements for short host defense peptides. With the help of a novel technique that uses a genetically created, luminescence-producing Gram-negative bacterium and peptide synthesis on cellulose, we investigated the sequence requirements for short host defense peptides. Thousands of peptides were tested for their ability to kill Pseudomonas aeruginosa. Complete substitution analyses of different Bac2A variants, as well as a semi-random peptide library with about 3000 members were studied. The complete substitution analysis provided information about the importance of each single amino acid position within a peptide sequence, while the peptide library offered broader information about amino acid composition resulting in an active antimicrobial peptide. The data were analyzed using a fuzzy logic quantitative structure-activity relationship approach (QSAR) to identify sequence requirements that discriminated among superior activity, equivalent activity and inactive peptides.
机译：关于短宿主防御肽的序列要求知之甚少。借助于使用遗传产生的发光革兰氏阴性细菌并在纤维素上合成肽的新技术，我们研究了短宿主防御肽的序列要求。测试了数千种肽杀死铜绿假单胞菌的能力。研究了不同Bac2A变体的完全取代分析，以及具有约3000个成员的半随机肽库。完整的取代分析提供了有关肽序列中每个单个氨基酸位置重要性的信息，而肽库提供了有关产生活性抗菌肽的氨基酸组成的更广泛信息。使用模糊逻辑定量结构-活性关系方法（QSAR）对数据进行分析，以识别区分出卓越活性，等效活性和非活性肽的序列要求。

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