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首页> 外文会议>WSEAS International Conference on Mathematical Biology and Ecology >Short cationic host defense peptides: determination of sequence requirements for killing Pseudomonas aeruginosa
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Short cationic host defense peptides: determination of sequence requirements for killing Pseudomonas aeruginosa

机译：短阳离子宿主防御肽：测定杀死假单胞菌铜绿假单胞菌的序列要求

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Little is known about sequence requirements for short host defense peptides. With the help of a novel technique that uses a genetically created, luminescence-producing Gram-negative bacterium and peptide synthesis on cellulose, we investigated the sequence requirements for short host defense peptides. Thousands of peptides were tested for their ability to kill Pseudomonas aeruginosa. Complete substitution analyses of different Bac2A variants, as well as a semi-random peptide library with about 3000 members were studied. The complete substitution analysis provided information about the importance of each single amino acid position within a peptide sequence, while the peptide library offered broader information about amino acid composition resulting in an active antimicrobial peptide. The data were analyzed using a fuzzy logic quantitative structure-activity relationship approach (QSAR) to identify sequence requirements that discriminated among superior activity, equivalent activity and inactive peptides.
机译：关于短宿主防御肽的序列要求，知之甚少。借助于使用遗传产生的产生遗传产生的发光的革兰氏阴性细菌和肽合成的新技术，研究了短宿主防御肽的序列要求。测试了数以千计的肽以杀死假单胞菌铜绿假单胞菌的能力。研究了不同BAC2A变体的完全替代分析，以及具有约3000个成员的半随机肽文库。完整的替代分析提供了关于肽序列中每种单一氨基酸位置的重要性的信息，而肽文库提供了有关氨基酸组合物的更广泛的信息，导致活性抗微生物肽。使用模糊逻辑定量结构 - 活性关系方法（QSAR）分析数据，以识别在优异的活性，等效活动和无活性肽之间区分的序列要求。

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