• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Light-based diagnosis and treatment of infections diseases >Photodynamic activity of natural anthraquinones on fibroblasts
【24h】

Photodynamic activity of natural anthraquinones on fibroblasts

机译：天然蒽醌对成纤维细胞的光动力活性

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Natural anthraquinones (AQs) isolated from Heterophyllaea lycioides (Rusby) Sandwith (Rubiaceae) demonstrated to have photodynamic properties: soranjididol (Sor), 5-Chlorosoranjidiol (5-ClSor). bisoranjidiol (Bisor). 7-Chlorobisoranjidiol (7-ClBisor) and lycionine (Lyc). Sor, 5-CISor and Bisor exhibited photodynamic inactivation on bacteria and parasites. As they could be used in topical application, the aim of this work was to study their photodynamic activity on fibroblasts. AQs were tested at 2.5 μM in darkness and under irradiation conditions. They were photoactivated with violet-blue LED (X = 410 ± 10 nm; fluence rate =50 mW/cm~2) and exposure time corresponded to a fluence of 27 J/cm~2. Negative and positive control (-C and +C, respectively) were included. Mitochondrial activity was determined by using MTT assay that is a measure of the cell viability and it was expressed as a percentage respect to -C (% CV). Results showed that AQs in darkness conditions showed similar metabolic activity as -C, except for 5-CISor (about 75% CV). Under irradiation, AQs exhibited dissimilar results. Sor and 7-ClBisor maintained cell viability at approximately 100%, Bisor and Lyc around 70%, whereas 5-CISor reduced cell viability by 90%. Taken together, our results suggest that Sor could mediate photodynamic therapy (PDT) in cutaneous infections since no toxicity was observed in fibroblasts. On the other hand, 5-CISor could be used for topical PDT of keloids and hypertrophic scars.
机译：从百里香（Rusby）Sandwith（Rubiaceae）分离得到的天然蒽醌（AQs）具有光动力学性质：茄基二醇（Sor），5-氯代呋喃二二醇（5-ClSor）。 bisoranjidiol（Bisor）。 7-氯异兰基二醇（7-ClBisor）和蛋氨酸（Lyc）。 Sor，5-CISor和Bisor对细菌和寄生虫表现出光动力学灭活作用。由于它们可用于局部应用，这项工作的目的是研究它们对成纤维细胞的光动力活性。在黑暗中和在辐射条件下以2.5μM测试AQ。它们用紫蓝色LED（X = 410±10 nm；注量速率= 50 mW / cm〜2）光活化，曝光时间对应于注量27 J / cm〜2。包括阴性和阳性对照（分别为-C和+ C）。通过使用MTT测定法测定线粒体活性，MTT测定法是细胞生存力的量度，并表示为相对于-C的百分比（％CV）。结果表明，在黑暗条件下，AQ的代谢活性与-C相似，除了5-CISor（约75％CV）。在辐射下，AQ表现出不同的结果。 Sor和7-ClBisor维持细胞活力约100％，Bisor和Lyc维持约70％，而5-CISor降低细胞活力90％。两者合计，我们的结果表明Sor可以介导皮肤感染中的光动力疗法（PDT），因为在成纤维细胞中未观察到毒性。另一方面，5-CISor可用于瘢痕loid和肥厚性瘢痕的局部PDT。

著录项

  • 来源
  • 会议地点 San Francisco(US)
  • 作者

    Jesica Dimmer; Camila Ramos Silva; Susana C. Nunez Montoya; Jose Luis Cabrera; Martha S. Ribeiro;

  • 作者单位

    IMBIV, CONICET, Dpto. de Cs. Farmaceuticas, Fac. Cs. Qcas. Universidad Nacional Cordoba. CP: X5000HUA. Cordoba, Argentina;

    LATO, CLA, Instituto Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP) - Av. Lineu Prestes 2242, Cidade Universitaria 'Armando de Salles Oliveira'- CEP 05508-000 - Sao Paulo - SP - Brasil;

    IMBIV, CONICET, Dpto. de Cs. Farmaceuticas, Fac. Cs. Qcas. Universidad Nacional Cordoba. CP: X5000HUA. Cordoba, Argentina;

    IMBIV, CONICET, Dpto. de Cs. Farmaceuticas, Fac. Cs. Qcas. Universidad Nacional Cordoba. CP: X5000HUA. Cordoba, Argentina;

    LATO, CLA, Instituto Pesquisas Energeticas e Nucleares (IPEN-CNEN/SP) - Av. Lineu Prestes 2242, Cidade Universitaria 'Armando de Salles Oliveira'- CEP 05508-000 - Sao Paulo - SP - Brasil;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    natural anthraquinones; photosensitizers; fibroblast; violet-blue LED;

    机译：天然蒽醌;光敏剂；成纤维细胞紫蓝色LED;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号