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首页> 外文会议>Biophotonics and immune responses X >Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry
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Noninvasive and label-free detection of circulating melanoma cells by in vivo photoacoustic flow cytometry

机译：体内光声流式细胞仪无创且无标记地检测循环黑素瘤细胞

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摘要

Melanoma is a malignant tumor of melanocytes. Circulating melanoma cell has high light absorption due to melanin highly contained in melanoma cells. This property is employed for the detection of circulating melanoma cell by in vivo photoacoustic flow cytometry (PAFC). PAFC is based on photoacoustic effect. Compared to in vivo flow cytometry based on fluorescence, PAFC can employ high melanin content of melanoma cells as endogenous biomarkers to detect circulating melanoma cells in vivo. In our research, we developed in vitro experiments to prove the ability of PAFC system of detecting PA signals from melanoma cells. For in vivo experiments, we constructed a model of melanoma tumor bearing mice by inoculating highly metastatic murine melanoma cancer cells, B16F10 with subcutaneous injection. PA signals were detected in the blood vessels of mouse ears in vivo. By counting circulating melanoma cells termly, we obtained the number variation of circulating melanoma cells as melanoma metastasized. Those results show that PAFC is a noninvasive and label-free method to detect melanoma metastases in blood or lymph circulation. Our PAFC system is an efficient tool to monitor melanoma metastases, cancer recurrence and therapeutic efficacy.
机译：黑色素瘤是黑色素细胞的恶性肿瘤。由于黑色素瘤细胞中高度含有黑色素，因此循环黑色素瘤细胞具有较高的光吸收率。此属性用于通过体内光声流式细胞术（PAFC）检测循环黑素瘤细胞。 PAFC基于光声效应。与基于荧光的体内流式细胞术相比，PAFC可以利用黑色素瘤细胞中高含量的黑色素作为内源性生物标志物来检测体内循环的黑色素瘤细胞。在我们的研究中，我们开发了体外实验来证明PAFC系统检测黑色素瘤细胞中PA信号的能力。对于体内实验，我们通过皮下注射接种高度转移的鼠类黑色素瘤癌细胞B16F10，构建了一个带有黑色素瘤荷瘤小鼠的模型。在体内在小鼠耳朵的血管中检测到PA信号。通过对循环中的黑色素瘤细胞进行长期计数，我们获得了随着黑色素瘤转移而变化的循环黑色素瘤细胞的数量变化。这些结果表明，PAFC是检测血液或淋巴循环中黑色素瘤转移的非侵入性且无标签的方法。我们的PAFC系统是监测黑色素瘤转移，癌症复发和治疗效果的有效工具。

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  • 来源
    《Biophotonics and immune responses X》|2015年|93240G.1-93240G.5|共5页
  • 会议地点 San Francisco CA(US)
  • 作者

    Ping Yang; Rongrong Liu; Zhenyu Niu; Yuanzhen Suo; Hao He; Xunbin Wei;

  • 作者单位

    Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China;

    Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China;

    Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China;

    Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China;

    Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China;

    Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    in vivo photoacoustic flow cytometry; circulating tumor cells; melanoma; B16F10 cell;

    机译：体内光声流式细胞术；循环肿瘤细胞；黑色素瘤B16F10细胞;

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