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首页> 外文会议>Conference on vaccine technology VI >PRODUCTION OF BACTERIAL OUTER MEMBRANE VESICLES AS VACCINE PLATFORM
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PRODUCTION OF BACTERIAL OUTER MEMBRANE VESICLES AS VACCINE PLATFORM

机译：疫苗平台生产细菌外膜囊泡

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Bacterial outer membrane vesicles (OMVs) are non-infectious but highly immunogenic particles. These vesicles are used as vaccines against the disease of the source bacteria. Fascinatingly, the addition of heterologous antigens to these vesicles creates a versatile vaccine platform. Such a platform can be used as an alternative to subunit vaccines, during infectious disease outbreaks or for the development of vaccines against pathogens that require high containment. A unique aspect of this platform is the reusability of the production process for many different vaccines. This in turn could reduce the time to market for new vaccines significantly. We designed a heterologous OMV vaccine concept for Lyme disease based on spontaneous released OMVs from Neisseria meningitidis that express the Outer surface protein A (OspA) of Borrelia burgdorferi on the surface. The productivity of spontaneously released OMVs was improved by the introduction of oxidative stress to the bacterial culture. Increased dissolved oxygen concentrations during cultivation showed to be an excellent process parameter for enhanced release of OMVs, while the bacterial culture remains viable. This presentation will cover the development of the OMV-based vaccine platform and the impact of changes in the upstream process on the downstream process of the investigational OMV-based Lyme disease vaccine.
机译：细菌外膜囊泡（OMV）是非感染性但具有高度免疫原性的颗粒。这些囊泡用作抵抗源细菌疾病的疫苗。令人着迷的是，向这些囊泡中添加异源抗原可创建通用的疫苗平台。这样的平台可以在传染病暴发期间用作亚单位疫苗的替代品，或用于开发针对需要高度遏制的病原体的疫苗。该平台的一个独特方面是许多不同疫苗在生产过程中的可重用性。反过来，这可以大大减少新疫苗的上市时间。我们基于脑膜炎奈瑟氏球菌自发释放的OMV设计了莱姆病的异源OMV疫苗概念，该表面表达了伯氏疏螺旋体的表面蛋白A（OspA）。通过向细菌培养物中引入氧化应激，可以提高自发释放的OMV的生产率。在培养过程中增加的溶解氧浓度显示出是增强OMV释放的极佳工艺参数，而细菌培养仍然可行。本演讲将介绍基于OMV的疫苗平台的开发以及上游过程中的变化对基于OMV的莱姆病研究疫苗的下游过程的影响。

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