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Effectiveness of tobramycin conjugated to iron oxide nanoparticles in treating infection in cystic fibrosis

机译:妥布霉素与氧化铁纳米粒子偶联治疗囊性纤维化感染的有效性

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Cystic fibrosis (CF) is an inherited childhood-onset life-shortening disease. It is characterized by increased respiratory production, leading to airway obstruction, chronic lung infection and inflammatory reactions. The most common bacteria causing persisting infections in people with CF is Pseudomonas aeruginosa. Superparamagnetic Fe_3O_4 iron oxide nanoparticles (NPs) conjugated to the antibiotic (tobramycin), guided by a gradient of the magnetic field or subjected to an oscillating magnetic field, show promise in improving the drug delivery across the mucus and P. aeruginosa biofilm to the bacteria. The question remains whether tobramycin needs to be released from the NPs after the penetration of the mucus barrier in order to act upon the pathogenic bacteria. We used a zero-length l-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) crosslinking agent to couple tobramycin, via its amine groups, to the carboxyl groups on Fe_3O_4 NPs capped with citric acid. The therapeutic efficiency of Fe_3O_4 NPs attached to the drug versus that of the free drug was investigated in P. aeruginosa culture.
机译:囊性纤维化(CF)是一种遗传性的儿童期缩短寿命的疾病。它的特点是增加呼吸,导致气道阻塞,慢性肺部感染和炎症反应。导致CF患者持续感染的最常见细菌是铜绿假单胞菌。在磁场梯度的引导下或经受振荡磁场的作用下,与抗生素(妥布霉素)结合的超顺磁性Fe_3O_4氧化铁纳米颗粒(NPs)显示出改善粘液和铜绿假单胞菌生物膜向细菌的药物输送的潜力。 。问题仍然在于,妥布霉素穿透粘液屏障后是否需要从NP中释放妥布霉素才能作用于致病菌。我们使用了零长度的1-乙基-3- [3-二甲基氨基丙基]碳二亚胺盐酸盐(EDC)交联剂,将妥布霉素通过其胺基与加有柠檬酸的Fe_3O_4 NP上的羧基偶联。在铜绿假单胞菌培养物中研究了与药物相连的Fe_3O_4 NPs与游离药物相比的治疗效果。

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