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Quantum-Dot Based Quantitative Identification of Pathogens in Complex Mixture

机译：基于量子点的复杂混合物病原菌定量鉴定

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摘要

In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-, and 100-mer target DNA with both probes was analyzed and quantified by flow cytometry and fluorescence microscopy on the scale of single particles. The following steps were used in the assay: (I) target selection by MP probes and (ii) target detection by QD probes. Hybridization efficiency between MP conjugated probes and target DNA hybrids was controlled by a fluorescent dye specific for nucleic acids. Fluorescence was detected by flow cytometry to distinguish differences in oligo sequences as short as 25-mer capturing in target DNA and by gel-electrophoresis in the case of QD probes. This report shows that effective manipulation and control of micro- and nanoparticles in hybridization assays is possible.
机译：在本研究中，我们描述了由磁性颗粒（MP）和量子点（QD）与目标DNA组成的夹心设计杂交探针，及其在检测禽流感病毒（H5N1）序列中的应用。用两种探针对25、40和100聚体目标DNA的杂交进行了分析，并通过流式细胞仪和荧光显微镜对单个颗粒的规模进行了定量。测定中使用以下步骤：（I）通过MP探针选择靶标和（ii）通过QD探针检测靶标。 MP共轭探针与目标DNA杂化物之间的杂交效率由对核酸特异的荧光染料控制。通过流式细胞仪检测荧光，以区分寡核苷酸序列的差异（在靶DNA中捕获至25个聚体），对于QD探针则通过凝胶电泳。该报告表明在杂交试验中有效控制和控制微米和纳米颗粒是可能的。

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  • 来源
    《Colloidal quantum dots for biomedical applications V》|2010年|P.75750M.1-75750M.11|共11页
  • 会议地点 San Francisco CA(US)
  • 作者

    Sun Hee Lim; Felix Bestvater; Philippe Buchy; Sek Mardy; Alexey Dan Chin Yu;

  • 作者单位

    Diagnostics, Institut Pasteur Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam City,Gyeonggi-do, Korea, 443-270 Center for Neglected Diseases Drug Discovery (CND3), Institut Pasteur Korea, Korea;

    Cell Biophysics, Institut Pasteur Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam City,Gyeonggi-do, Korea, 443-270;

    Virology Unit, Institut Pasteur in Cambodia, 5, Monivong boulevard, Phnom Penh,Cambodia;

    Virology Unit, Institut Pasteur in Cambodia, 5, Monivong boulevard, Phnom Penh,Cambodia;

    Diagnostics, Institut Pasteur Korea, 696 Sampyeong-dong, Bundang-gu, Seongnam City,Gyeonggi-do, Korea, 443-270 Department of Nanoscience and Technology, Korean Advanced Institute of Science and Technology (KAIST), Daejeon, Korea, 305-701;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
  • 关键词

    magnetic particles; quantum dots; hybridization; DNA; flow cytometry; fluorescence microscopy;

    机译：磁性粒子量子点;杂交脱氧核糖核酸;流式细胞仪荧光显微镜;

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