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首页> 外文会议>SPIE Conference on Colloidal Quantum Dots for Biomedical Applications >Quantum-Dot Based Quantitative Identification of Pathogensin Complex Mixture
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Quantum-Dot Based Quantitative Identification of Pathogensin Complex Mixture

机译：基于量子的病原体复合物的定量鉴定

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In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-, and 100-mer target DNA with both probes was analyzed and quantified by flow cytometry and fluorescence microscopy on the scale of single particles. The following steps were used in the assay: (i) target selection by MP probes and (ii) target detection by QD probes. Hybridization efficiency between MP conjugated probes and target DNA hybrids was controlled by a fluorescent dye specific for nucleic acids. Fluorescence was detected by flow cytometry to distinguish differences in oligo sequences as short as 25-mer capturing in target DNA and by gel-electrophoresis in the case of QD probes. This report shows that effective manipulation and control of micro- and nanoparticles in hybridization assays is possible.
机译：在本研究中，我们描述了由磁性粒子（MP）和量子点（QD）组成的夹层设计杂交探针，其具有靶DNA，以及它们在检测禽流感病毒（H5N1）序列中的应用。通过流式细胞术和荧光显微镜在单颗粒的规模上分析和定量具有两个探针的25-，40-和100mer靶DNA的杂交。在测定中使用以下步骤：（i）MP探针的靶选择和（ii）QD探针的目标检测。 MP缀合探针和靶DNA杂交物之间的杂交效率由对核酸特异的荧光染料控制。通过流式细胞术检测荧光以区分低至QD探针的靶DNA中的寡核苷酸序列的差异，并通过QD探针的情况下通过凝胶电泳。本报告表明，杂交测定中的微型和纳米颗粒的有效操纵和控制是可能的。

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