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Studying nanotoxic effects of CdTe Quantum Dots in Trypanosoma cruzi

机译：研究CdTe量子点在克氏锥虫中的纳米毒性作用

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摘要

Many studies have been done in order to verify the possible nanotoxicity of quantum dots in some cellular types. Protozoan pathogens as Trypanosoma cruzi, etiologic agent of Chagas' disease is transmitted to humans either by blood-sucking triatomine vectors, blood transfusion, organs transplantation or congenital transmission. The study of the life cycle, biochemical, genetics, morphology and others aspects of the T. cruzi is very important to better understand the interactions with its hosts and the disease evolution on humans. Quantum dot, nanocrystals, highly luminescent has been used as tool for experiments in in vitro and in vivo T. cruzi life cycle development in real time. We are now investigating the quantum dots toxicity on T. cruzi parasite cells using analytical methods. In vitro experiments were been done in order to test the interference of this nanoparticle on parasite development, morphology and viability (live-death). Ours previous results demonstrated that 72 hours after parasite incubation with 200 uM of CdTe altered the development of T. cruzi and induced cell death by necrosis in a rate of 34%. QDs labeling did not effect: (I) on parasite integrity, at least until 7 days; (ii) parasite cell dividing and (iii) parasite motility at a concentration of 2 uM CdTe. This fact confirms the low level of cytotoxicity of these QDs on this parasite cell. In summary our results is showing T. cruzi QDs labeling could be used for in vivo cellular studies in Chagas disease.
机译：为了验证某些细胞类型中量子点的可能的纳米毒性，已经进行了许多研究。原生动物病原体如锥虫锥虫，恰加斯氏病的病原体，是通过吸血三氢番茄红素载体，输血，器官移植或先天性传播而传播给人类的。对克鲁斯锥虫的生命周期，生化，遗传，形态和其他方面的研究对于更好地了解与其宿主之间的相互作用以及人类疾病的发展非常重要。量子点，纳米晶体，高发光度已被用作实时体外和体内克鲁氏锥虫生命周期发展实验的工具。我们现在正在使用分析方法研究克氏锥虫寄生细胞的量子点毒性。为了测试该纳米粒子对寄生虫发育，形态和生存力（活死）的干扰，进行了体外实验。我们以前的研究结果表明，与200 uM的CdTe一起温育寄生虫后72小时改变了克鲁氏锥虫的发育并通过坏死诱导细胞死亡，发生率高达34％。 QD标记不起作用：（I）至少在7天之前对寄生虫的完整性产生影响； （ii）寄生虫细胞分裂和（iii）浓度为2 uM CdTe的寄生虫运动。这一事实证实了这些量子点对这种寄生虫细胞的细胞毒性水平很低。总而言之，我们的结果表明，克鲁氏锥虫QDs标记可用于南美锥虫病的体内细胞研究。

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  • 来源
  • 会议地点 San Francisco CA(US)
  • 作者

    C. V. Stahl; D. B. Almeida; A. A. de Thomaz; A. Fontes; R.F.S. Menna-Barreto; J. R. Santos-Mallet; C. L. Cesar; S. A.O. Gomes; D. Feder;

  • 作者单位

    Laboratorio de Transmissores de Leishmanioses, Setor de Morfologia, Ultraestrutura e Bioquimica de Artropodcs e Parasitos, IOC-FIOCRUZ-Rio de Janeiro, RJ, Brazil;

    Laboratorio de Aplicacoes Biomedicas de Lasers, Instituto de Fisica 'Gleb Wataghin', UNICAMP, Campinas, SP,Brazil;

    Laboratorio de Aplicacoes Biomedicas de Lasers, Instituto de Fisica 'Gleb Wataghin', UNICAMP, Campinas, SP,Brazil;

    Depto de Biofisica e Radiobiologia, CCB, UFPE, Recife, PE, Brazil;

    Laboratorio de Biologia Celular, IOC-FIOCRUZ-Rio dc Janeiro, RJ, Brazil;

    Laboratorio de Transmissores de Leishmanioses, Setor de Morfologia, Ultraestrutura e Bioquimica de Artropodcs e Parasitos, IOC-FIOCRUZ-Rio de Janeiro, RJ, Brazil;

    Laboratorio de Aplicacoes Biomedicas de Lasers, Instituto de Fisica 'Gleb Wataghin', UNICAMP, Campinas, SP,Bra;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医用物理学;
  • 关键词

    CdTe quantum dots trypanosoma cruzi nanotoxicity;

    机译：CdTe量子点锥虫克鲁兹纳米毒性;

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