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首页> 外文期刊>Memórias do Instituto Oswaldo Cruz >Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi
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Studying nanotoxic effects of CdTe quantum dots in Trypanosoma cruzi

机译：研究CdTe量子点对克鲁氏锥虫的纳米毒性作用

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Semiconductor nanoparticles, such as quantum dots (QDs), were used to carry out experiments in vivo and ex vivo with Trypanosoma cruzi. However, questions have been raised regarding the nanotoxicity of QDs in living cells, microorganisms, tissues and whole animals. The objective of this paper was to conduct a QD nanotoxicity study on living T. cruzi protozoa using analytical methods. This was accomplished using in vitro experiments to test the interference of the QDs on parasite development, morphology and viability. Our results show that after 72 h, a 200 μM cadmium telluride (CdTe) QD solution induced important morphological alterations in T. cruzi, such as DNA damage, plasma membrane blebbing and mitochondrial swelling. Flow cytometry assays showed no damage to the plasma membrane when incubated with 200 μM CdTe QDs for up to 72 h (propidium iodide cells), giving no evidence of classical necrosis. Parasites incubated with 2 μM CdTe QDs still proliferated after seven days. In summary, a low concentration of CdTe QDs (2 μM) is optimal for bioimaging, whereas a high concentration (200 μM CdTe) could be toxic to cells. Taken together, our data indicate that 2 μM QD can be used for the successful long-term study of the parasite-vector interaction in real time.
机译：半导体纳米粒子，例如量子点（QDs），被用于进行克鲁氏锥虫的体内和离体实验。然而，已经提出了关于QD在活细胞，微生物，组织和整个动物中的纳米毒性的问题。本文的目的是使用分析方法对活的克鲁氏原生动物原生动物进行QD纳米毒性研究。这是通过使用体外实验来测试QD对寄生虫发育，形态和生存力的干扰而完成的。我们的结果表明，在72 h后，200μM碲化镉（CdTe）QD溶液诱导了克鲁氏锥虫的重要形态变化，例如DNA损伤，质膜起泡和线粒体肿胀。流式细胞仪检测显示，与200μMCdTe QD孵育长达72小时（碘化丙啶细胞）后，质膜没有受到损坏，没有经典坏死的迹象。与2μMCdTe QD孵育的寄生虫在7天后仍会增殖。总之，低浓度的CdTe QD（2μM）最适合生物成像，而高浓度（200μMCdTe）可能对细胞有毒。综上所述，我们的数据表明2μMQD可用于实时成功地长期研究寄生虫-载体相互作用。

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