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首页> 外文会议>Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II; 20040127-20040128; San Jose,CA; US >Single molecule spectroscopy to probe competitive fluorescence resonance energy transfer pathways in bichromophoric synthetic systems
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Single molecule spectroscopy to probe competitive fluorescence resonance energy transfer pathways in bichromophoric synthetic systems

机译：单分子光谱法研究双发色合成系统中竞争性荧光共振能量转移途径

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摘要

Using single molecule fluorescence spectroscopy we have investigated fluorescence resonance energy transfer (FRET) occurring between two peryleneimide (PI) chromophores in a series of synthetic systems: PI end-capped fluorene trimers, hexamers and polymers for which the interchromophoric distance vary from 3.4 to 5.9 and 42 nm, respectively. By monitoring in parallel the fluorescence intensity and the number of independent emitting chromophores from each molecule, we could discriminate between competitive Foerster-type energy transfer processes such as energy hopping, singlet-singlet annihilation and singlet-triplet annihilation for the PI end-capped fluorine compounds. Due to different energy transfer efficiencies, variations in the interchromophoric distance enable switching between these processes. The single molecule fluorescence data reported here suggest that similar energy transfer pathways have to be considered in the analysis of single molecule trajectories of donor/acceptor pairs, as well as in the case of more complex systems like natural multichromophoric systems, such as light harvesting antennas or oligomeric fluorescent proteins.
机译：我们使用单分子荧光光谱法研究了一系列合成系统中两个：酰亚胺（PI）生色团之间发生的荧光共振能量转移（FRET）：PI端基芴三聚体，六聚体和聚合物，其发色间距离在3.4至5.9之间变化和42 nm。通过并行监测每个分子的荧光强度和独立发光生色团的数量，我们可以区分竞争性的Foerster型能量转移过程，例如能量跳跃，单峰-单态an灭和单峰-三重态an灭PI封端的氟化合物。由于不同的能量传递效率，发色间距离的变化使得能够在这些过程之间进行切换。此处报道的单分子荧光数据表明，在分析供体/受体对的单分子轨迹时，以及在更复杂的系统（例如天然多发色系统）（例如光收集天线）的情况下，必须考虑类似的能量转移途径或寡聚荧光蛋白。

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