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首页> 外文会议>Conference on Lasers in Dentistry Ⅶ Jan 21-22, 2001, San Jose, USA >CO_2-laser biopsies of oral mucosa: an immunocytological and histological comparative study
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CO_2-laser biopsies of oral mucosa: an immunocytological and histological comparative study

机译：口腔粘膜CO_2激光活检：免疫细胞学和组织学比较研究

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摘要

The relationship between bioptic technique and tissue preservation has been studied in 18 oral biopsies of young patients obtained by electrosurgery or CO_2 laser surgery. Biopsies were formalin fixed, paraffin embedded and histologically, histochemically and immunocytochemically (SBS-DAB method) treated. All the biopsies show inflammatory cell infiltration, epithelial spongiosis, trichocariosis, suprabasal small blisters, and epithelial clefts with lamina detaching from the corium. Histochemistry shows both the presence of edema and acid mucopolysaccharides inside the corium, and variable glycogen content in epithelial cells. Trichocariotic cells show a positive MiBl/Ki67 expression, when they are present. Nevertheless, laser biopsies show a lower amount of basophilic fibrous tissue and of bc12 bodies detection, connected with a higher amount of glycogen, Cytokeratin and MiBl/Ki67 expression in epithelial cells, compared to bovie biopsies. The results show a higher degree of damages in particular at the epithelial level, in electrosurgery biopsies rather than laser biopsies. The best epithelial and corium preservation showed by laser biopsies suggest a chance of reversible condition, which can lead to a complete recovery due to its higher capability of restoring tissues.
机译：活检技术与组织保存之间的关系已在通过电外科或CO_2激光手术获得的18例年轻患者的口腔活检中进行了研究。活检标本经福尔马林固定，石蜡包埋，组织学，组织化学和免疫细胞化学（SBS-DAB法）处理。所有活检均显示炎性细胞浸润，上皮海绵样变，毛滴虫病，基底上小水泡和上皮裂隙，其中层板从皮质脱落。组织化学显示皮质中存在水肿和酸性粘多糖，并且上皮细胞中糖原含量可变。当存在时，毛滴虫细胞显示出阳性的MiB1 / Ki67表达。然而，与牛活检相比，激光活检显示出较少量的嗜碱性纤维组织和bc12体检测，与上皮细胞中较高数量的糖原，细胞角蛋白和MiB1 / Ki67表达有关。结果显示，在电外科活检而不是激光活检中，尤其是在上皮水平的损伤程度更高。激光活检显示最好的上皮和真皮保存表明存在可逆性疾病的机会，由于它具有更高的恢复组织能力，可以导致完全恢复。

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