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首页> 外文会议>Conference on Optical Technologies in Biophysics and Medicine IV; Oct 1-4, 2002; Saratov, Russia >NIR Autofluorescence and OCT Imaging of Biotissues
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NIR Autofluorescence and OCT Imaging of Biotissues

机译：生物组织的近红外自发荧光和OCT成像

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Optical coherence tomography (OCT) is one of the most promising recently developed methods for non-invasive in vivo characterisation of biological highly scattering tissues. However, one of the drawbacks of the pure OCT imaging is that it is not sensitive to changes in metabolism. This may impact derived information and consecutive diagnostics, because pathological changes of tissue structure are accompanied with changes in metabolic activity or functional state in these areas even if there is not yet strongly evident structural change. Therefore, it is desirable to combine early detection of tissue malformations by OCT with other techniques, capable to detect and evaluate their functional state. One of suitable candidates for such non-invasive optical functional imaging is detection of laser induced autofluorescence, which could provide information about rate of biological and chemical processes in living cells. As an example, the cells in proliferative state (with increased metabolic activity or during mitosis) show more intensive NIR fluorescence than the cells that are not proliferative, because of increased concentration of free porphyrins. In presented study we used OCT and laser induced NIR autofluorescence imaging for detection and evaluation of changes in areas of naevus and injuries in group of volunteers. The fusion of information on structural and functional state of biotissue provided by the two mentioned complementary methods may enhance the diagnostics power of their prospective clinical use. Firstly the fluorescence of area of naevus and injuries, excited by 630 nm was taken by CCD camera and then was naevus scanned by OCT. The findings of both methods were compared and correlated. In the case of naevus the obtained results were reviewed with histological treatment of the same area.
机译：光学相干断层扫描（OCT）是最近开发的最有前途的方法，用于对生物高度散射的组织进行非侵入式体内表征。但是，纯OCT成像的缺点之一是它对新陈代谢的变化不敏感。这可能会影响衍生信息和连续诊断，因为组织结构的病理变化伴随着这些区域的代谢活性或功能状态的变化，即使还没有很明显的结构变化。因此，期望将通过OCT对组织畸形的早期检测与能够检测和评估其功能状态的其他技术相结合。这种无创光学功能成像的合适候选者之一是检测激光诱导的自发荧光，它可以提供有关活细胞中生物和化学过程速率的信息。例如，由于游离卟啉的浓度增加，处于增殖状态（具有增加的代谢活性或在有丝分裂期间）的细胞显示出比非增殖细胞更强的NIR荧光。在提出的研究中，我们使用OCT和激光诱导的NIR自发荧光成像来检测和评估志愿者组中痣和损伤区域的变化。通过上述两种互补方法提供的有关生物组织的结构和功能状态的信息融合可以增强其预期临床用途的诊断能力。首先用CCD相机对630 nm激发的痣和损伤部位的荧光进行采集，然后用OCT扫描痣。比较并关联了这两种方法的发现。在痣的情况下，通过相同区域的组织学治疗对获得的结果进行回顾。

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