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Optical molecular imaging

机译:光学分子成像

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摘要

Planar illumination techniques offer significant utility in probing optical contrast at tissue surfaces. For probing events deeper in tissue the development of more advanced imaging methods may be required to obtain quantitative and three-dimensional localization. In view of recent developments of novel biocompatible fluorescent probes for probing molecular function, optical imaging methods may be used towards non-invasive molecular imaging in-vivo with high specificity and localization accuracy. Underpinnings to these developments are the use of near infrared photons to allow deep penetration in tissues, highly sensitive photon detection technologies and appropriate theories and mathematical tools for tomographic reconstruction of optical signals. Herein we briefly outline key components of optical imaging in planar and tomographic mode as an investigation tool for carcinogenesis and focus on recent progress with optical tomography of tissues. Imaging potential and limitations for molecular and clinical imaging are also discussed.
机译:平面照明技术在探测组织表面的光学对比度方面提供了显着的实用性。为了探测组织中更深处的事件,可能需要开发更先进的成像方法以获得定量和三维定位。鉴于用于探测分子功能的新型生物相容荧光探针的最新发展,光学成像方法可用于具有高特异性和定位精度的体内非侵入性分子成像。这些发展的基础是使用近红外光子以允许在组织中的深入渗透,高度灵敏的光子检测技术以及用于光信号的层析重建的适当理论和数学工具。在此,我们简要概述了平面成像和断层扫描模式下光学成像的关键组成部分,作为致癌作用的研究工具,并着重介绍了组织断层成像的最新进展。还讨论了成像潜力以及分子和临床成像的局限性。

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