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首页> 外文会议>Conference on Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, Oct 15-18, 2002, Shanghai, China >Rapid and sensitive immunomagnetic-electrochemiluminescent detection of P53 protein from human lung cancer cell
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Rapid and sensitive immunomagnetic-electrochemiluminescent detection of P53 protein from human lung cancer cell

机译：快速灵敏的免疫磁化学发光法检测人肺癌细胞中的P53蛋白

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In this work, we have designed an electrochemiluminescence (ECL) reaction system used to indicate P53 protein from human lung cancer cells. And we optimized the working electrode firstly in this experiment. The electrochemiluminescence magnetic immunoassay is quantitative, reproducible, and requires only minimal sample processing. A single photon counter was used to heighten the ECL detection limit. The homologous immunoassay format consisted of a double antibody sandwich in which a biotinylated capture antibody, pre-bound to streptavidin-coated magnetic beads, was used to bind antigen from test samples. A detector antibody, labeled with ruthenium(Ⅱ) tris-bipyridal chelate, was added and, when bounded to the bead immunocomplex, light was generated in the presence of an excess of tripropylamine. The light was detected and measured by the ECL reaction system we designed. High-sensitive, rapid and reproducible detection of tumor marker P53 protein was actualize with this detection system. The results showed .that the electrode with many holes on it can enhance the ECL efficiency, so the detection limit of the system can be improved to 1/1000 fmol/L free labeled Ru(bpy)_3~(2+). Ru(bpy)_3~(2+) + TPA(tripropylmine) ECL reaction system was used to indicate P53 protein from human lung cancer cells. This method could have potential applications in early-stage tumor diagnosis and environmental inspection.
机译：在这项工作中，我们设计了一种电化学发光（ECL）反应系统，用于指示人肺癌细胞中的P53蛋白。并且我们在该实验中首先优化了工作电极。电化学发光磁免疫测定法是定量的，可重复的，并且仅需要最少的样品处理。使用单个光子计数器来提高ECL检测极限。同源免疫测定法由双抗体夹心组成，其中将生物素化的捕获抗体预先结合到链霉亲和素包被的磁珠上，以结合来自测试样品的抗原。加入标记有钌（Ⅱ）三联吡啶鎓螯合物的检测抗体，当与珠免疫复合物结合时，在过量的三丙胺存在下会发光。通过我们设计的ECL反应系统检测并测量了光。使用该检测系统实现了对肿瘤标志物P53蛋白的高灵敏，快速和可重复的检测。结果表明，带有多个孔的电极可以提高ECL效率，因此该系统的检测限可以提高到1/1000 fmol / L游离标记的Ru（bpy）_3〜（2+）。用Ru（bpy）_3〜（2+）+ TPA（三丙胺）ECL反应系统指示人肺癌细胞中的P53蛋白。该方法在早期肿瘤诊断和环境检查中可能具有潜在的应用价值。

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