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Application of Modern Techniques for Studying Bacterial Pathogens in Georgia

机译：现代技术在佐治亚州细菌致病菌研究中的应用

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Over the last few years several molecular methods for rapid identification and fingerprinting of different pathogens have been applied at the National Center for Disease Control and Public health of Georgia (NCDC). These modern techniques strengthen the NCDC Georgia laboratory's capabilities to rapidly respond to urgent public health threats and assist in the detection and tracking of these diseases. Application of genotyping techniques for investigation of NCDC Live Culture Collections enables Georgian public health scientists to determine the extent of strain variability as well as define the ability of new molecular techniques to characterize potential biothreat organisms circulating in the wild. Molecular methods, such as pulsed-field gel electrophoresis (PFGE), IS (insertion sequence) element fingerprinting, multiple-locus variable number tandem repeat analysis (MLVA) have been applied at NCDC in order to characterize bacterial isolates of Yersinis pestis and Francisella tularensis obtained from a variety of different foci. Such characterization has helped close a significant gap in our knowledge of strains present in this geographical region.
机译：在过去的几年中，乔治亚州国家疾病控制和公共卫生中心（NCDC）已采用了几种用于快速识别和鉴定不同病原体的分子方法。这些现代技术增强了NCDC佐治亚州实验室的能力，可以迅速应对紧急公共卫生威胁并协助发现和跟踪这些疾病。基因分型技术在NCDC活培养物收集研究中的应用使格鲁吉亚公共卫生科学家能够确定菌株变异的程度，并定义新分子技术表征野外传播的潜在生物威胁生物的能力。分子方法，如脉冲场凝胶电泳（PFGE），IS（插入序列）元素指纹分析，多位点可变数目串联重复分析（MLVA）已在NCDC上应用，以鉴定鼠疫耶尔森氏菌和土拉弗朗西斯菌的细菌分离物。从各种不同的病灶获得。这种表征有助于弥补我们对这个地理区域中存在的菌株的认识上的巨大空白。

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