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首页> 外文会议>Energy-based treatment of tissue and assessment VIII >Tissue healing response following hyperthermic vapor ablation in the porcine longissimus muscle
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Tissue healing response following hyperthermic vapor ablation in the porcine longissimus muscle

机译：猪纵长肌高温蒸气消融后的组织愈合反应

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摘要

As the use of hyperthermic ablation technologies has increased, so too has the need to understand their effects on tissue and their healing responses. This study was designed to characterize tissue injury and healing following hyperthermic vapor ablation in the in vivo porcine longissimus muscle model. The individual ablations were performed using the NxThera Vapor Delivery System (NxThera Inc., Minneapolis, MN). To assess the vapor ablation's evolution, the swine were euthanized post-treatment on Day 0, Day 3, Day 7, Day 14, Day 28, Day 45 and Day 90. Triphenyltetrazolium chloride viability staining (TTC staining) was used to macroscopically assess the extent of each vapor ablation within the tissue. The ablation associated healing responses were then histologically evaluated for acute inflammation, chronic inflammation, foreign body reaction and fibrosis. Two zones of tissue injury were initially identified in the ablations: 1) a central zone of complete coagulative necrosis and 2) an outer "transition zone" of viable and non-viable cells. The ablations initially increased in size from Day 0 to Day 7 and then progressively decreased in size though Day 45. The initial Day 3 healing changes originated in the transition zone with minimal acute and chronic inflammation. As time progressed, granulation tissue began to form by Day 7 and peaked around Day 14. Collagen formation, deposition and remodeling began in the adjacent healthy tissue by Day 28, replaced the ablation site by Day 45 and reorganized by Day 90. In conclusion, this vapor ablation technology provided a non-desiccating form of hyperthermic ablation that resulted in coagulative necrosis without a central thermally/heat-fixed tissue component, followed a classical wound healing pathway, and healed with minimal associated inflammation.
机译：随着高温消融技术的使用增加，也需要了解其对组织的影响及其愈合反应。这项研究旨在表征体内猪最长肌肌肉模型中高温蒸气消融后的组织损伤和愈合。使用NxThera蒸汽输送系统（明尼苏达州明尼阿波利斯的NxThera Inc.）进行个体消融。为了评估蒸汽消融的演变，在处理后的第0天，第3天，第7天，第14天，第28天，第45天和第90天对猪进行了安乐死。使用氯化三苯四唑活度染色（TTC染色）宏观评估组织内每种蒸汽消融的程度。然后在组织学上评估与消融相关的愈合反应是否为急性炎症，慢性炎症，异物反应和纤维化。在消融术中最初确定了两个组织损伤区域：1）完全凝固性坏死的中央区域，以及2）活细胞和非活细胞的外部“过渡区”。消融术最初从第0天到第7天大小增加，然后到第45天逐渐减小。最初的第3天愈合变化起源于过渡区，急性和慢性炎症最小。随着时间的流逝，肉芽组织在第7天开始形成，并在第14天达到峰值。到28天，相邻健康组织中的胶原蛋白形成，沉积和重塑开始，在45天开始取代消融位点，并在90天重新组织。这种蒸气消融技术提供了一种非干燥形式的高温消融，可导致凝固性坏死，而没有中央的热/热固定组织成分，遵​​循经典的伤口愈合途径，并以最小的相关炎症得到治愈。

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  • 会议地点 San Francisco CA(US)
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    John T. Grantham; Brian T. Grisez; Justin Famoso; Michael Hoey; Chris Dixon; James E. Coad;

  • 作者单位

    Pathology Laboratory for Translational Medicine, West Virginia University, PO Box 9203, Morgantown, WV, USA 26506-9203;

    Pathology Laboratory for Translational Medicine, West Virginia University, PO Box 9203, Morgantown, WV, USA 26506-9203;

    Pathology Laboratory for Translational Medicine, West Virginia University, PO Box 9203, Morgantown, WV, USA 26506-9203;

    NxThera, 7351 Kirkwood Lane North, Suite 138, Maple Grove, MN, USA 55369-5219;

    Department of Urology, New York Medical College, 40 Sunshine Cottage Rd, Skyline 1S-B48, Valhalla, NY, USA 10595-1524;

    Pathology Laboratory for Translational Medicine, West Virginia University, PO Box 9203, Morgantown, WV, USA 26506-9203;

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