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PURIFICATION AND BIOACTIVITY DETERMINATION OF HGF FROM NEW-BORN COW LIVER

机译:新生牛肝中HGF的纯化和生物活性测定

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摘要

Hepatocyte growth factor (HGF) was a heparin-binding glycoprotein which could stimulate strongly hepatocytes division [1,2,3]. During the past ten years, many biologists had done a lot of works about HGF [4,5,6]. But the heparin -sepharose gel (made by Pharmacia Company) is very expensive, so the purification of HGF in large amounts is limited. In this paper, using heparin -sepharose 4B (self-prepared) chromatography as the first-step, we purified the HGF from new-born cow liver and determined its bioactivity.
机译:肝细胞生长因子(HGF)是一种肝素结合糖蛋白,可以强烈刺激肝细胞分裂[1,2,3]。在过去的十年中,许多生物学家做了很多有关HGF的工作[4,5,6]。但是,肝素-琼脂糖凝胶(Pharmacia Company制造)非常昂贵,因此大量纯化HGF受到限制。在本文中,以肝素-琼脂糖4B(自备)色谱为第一步,我们从新生牛肝中纯化了HGF,并确定了其生物活性。

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