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Advances in photon counting for bioluminescence

机译:生物发光的光子计数研究进展

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Abstract: Photon counting systems were originally developed for astronomy, initially by the astronomical community. However, a major application area is in the study of luminescent probes in living plants, fishes and cell cultures. For these applications, it has been necessary to develop camera system capability at very low light levels - a few photons occasionally - and also at reasonably high light levels to enable the systems to be focused and to collect quality images of the object under study. The paper presents new data on MTF at extremely low photon flux and conventional ICCD illumination, counting efficiency and dark noise as a function of temperature. !0
机译:摘要:光子计数系统最初是为天文学开发的,最初是由天文学界开发的。但是,主要的应用领域是在活植物,鱼类和细胞培养物中研究发光探针。对于这些应用,必须在非常低的光照水平下(偶尔有几个光子)并且在相当高的光照水平下发展相机系统的能力,以使系统能够聚焦并收集研究对象的高质量图像。本文介绍了在极低光子通量和常规ICCD照明下MTF的新数据,并计算了效率和暗噪声随温度的变化。 !0

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