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Advances in photon counting for bioluminescence

机译:对生物发光计数的光子进展

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摘要

Photon counting systems were originally developed for astronomy, initially by the astronomical community. However, a major application area is in the study of luminescent probes in living plants, fishes and cell cultures. For these applications, it has been necessary to develop camera system capability at very low light levels - a few photons occasionally - and also at reasonably high light levels to enable the systems to be focused and to collect quality images of the object under study. The paper presents new data on MTF at extremely low photon flux and conventional ICCD illumination, counting efficiency and dark noise as a function of temperature.
机译:最初由天文社区最初用于天文学的光子计数系统。然而,主要的应用领域正在研究生物植物,鱼类和细胞培养物中的发光探针。对于这些应用,有必要在非常低的光线水平下开发相机系统能力 - 偶尔的几个光子 - 并且还具有相当高的光线水平,以使得系统能够聚焦并收集正在研究的物体的质量图像。本文以极低的光子通量和传统的ICCD照明,计数效率和暗噪声作为温度的函数,呈现MTF上的新数据。

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