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MEM-FLIM: all-solid-state camera for fluorescence lifetime imaging

机译:MEM-FLIM:用于荧光寿命成像的全固态摄像机

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We have built an all-solid-state camera which is directly modulated at the pixel level for frequency domain fluorescence lifetime imaging microscopy (FLIM) measurement. This novel camera eliminates the need for an image intensifier through the use of an application-specific CCD design, which is being used in a frequency domain FLIM system. The first stage of evaluation for the camera has been carried out. Camera characteristics such as noise distribution, dark current influence, camera gain, sampling density, sensitivity, linearity of photometric response, and contrast modulation transfer function have been studied through experiments. We are able to do lifetime measurement using MEM-FLIM cameras for various objects, e.g. fluorescence plastic test slides, fluorescein solution, fixed GFP cells, and GFP - Actin stained live cells.
机译:我们建立了一个全固态摄像头,该摄像头直接在像素级进行了调制,用于频域荧光寿命成像显微镜(FLIM)测量。通过使用专用CCD设计,这种新颖的相机消除了对图像增强器的需求,该CCD设计已在频域FLIM系统中使用。相机评估的第一阶段已经完成。通过实验研究了诸如噪声分布,暗电流影响,照相机增益,采样密度,灵敏度,光度响应线性和对比度调制传递函数等照相机特性。我们能够使用MEM-FLIM相机对各种物体进行寿命测量,例如荧光塑料测试玻片,荧光素溶液,固定的GFP细胞和GFP-肌动蛋白染色的活细胞。

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