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首页> 外文会议>Instruments, methods, and missions for astrobiology XVII >Molecular studies of anaerobic strains from Antarctica and their taxonomic identification
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Molecular studies of anaerobic strains from Antarctica and their taxonomic identification

机译：南极厌氧菌的分子研究及其分类学鉴定

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摘要

We present phylogenetic analyses for four anaerobic bacterial isolates from samples collected in the Schirmacher Oasis and Lake Untersee in Antarctica. Near-full length of 16S rRNA genes were amplified from the four strains and sequenced for identification of their close relatives and their phylogenetic relationships. Strain A7P-90m shared a low 16S rRNA sequence identity of around 85% with its closest relatives within the Bacteroides phylum. This low level of sequence similarity suggests that it may represent a novel family within this phylum. The 16S rRNA sequence identity between strain LZ-22 and its closest relatives Granulicoccus phenolivorans and Propioniferax innocua within the Propionibacteriaceae family were 91.9% and 93.2%, respectively. This low level of sequence similarity suggests that it may represent a novel genus within this family. Strains 9G and ISLP-3 were closely related to known species of the genera Halolactibacillus and Sanguibacter, respectively. However, the 16S rRNA sequence identities between strains 9G and ISLP-3 and their close relatives were too high to make reliable taxonomic inferences (i.e., 99.9% between 9G and H. miurensis, and 98.6% between ISLP-3 and S. suaresii). Because the recA gene delivers higher resolution for taxonomic inferences than the 16S rRNA gene, the primers for conserved recA gene were designed for PCR amplification and sequencing from Halolactibacillus and Sanguibacter type strains. Strain 9G shared a recA sequence identity of 99.6% with its closest relative H. miurensis, suggesting that it is a subspecies. The recA sequence identity shared between strain ISLP-3 and its six closest relatives ranged from 85.9～90.2%. This result is consistent with this strain representing a novel species within the genus Sanguibacter. Based on the molecular study presented here and the phenotypic properties presented elsewhere, we propose that strain LZ-22 is a representative of a novel genus and species, with proposed names Raineyella antarctica gen. nov., sp. nov. Strain ISLP-3 is a representative of a novel species, Sanguibacter gelidistatuaria sp. nov. Strain A7p-90m may represent a novel family within the order Bacteroidales. Chemotaxonomic characterizations of these strains are underway to gather more evidence for the proposed classifications.
机译：我们目前对南极Schirmacher绿洲和Untersee湖中收集的样本中的四种厌氧细菌分离株进行系统发育分析。从这四个菌株中扩增出近乎全长的16S rRNA基因，并对其进行测序，以鉴定其近亲和亲缘关系。 A7P-90m菌株与其最接近的亲缘动物共有16S rRNA序列同一性低，约为85％。这种低水平的序列相似性表明它可能代表了这个门上的一个新家族。丙酸杆菌科中的菌株LZ-22与其最接近的近亲酚颗粒葡萄球菌和无性丙酸杆菌之间的16S rRNA序列同一性分别为91.9％和93.2％。这种低水平的序列相似性表明它可能代表了这个家族中的一个新属。 9G和ISLP-3菌株分别与嗜盐乳杆菌和Sanguibacter属的已知物种密切相关。但是，菌株9G和ISLP-3及其近亲之间的16S rRNA序列同一性太高，无法做出可靠的分类学推论（即9G和miurensis之间的99.9％，ISLP-3和suaresii之间的98.6％）。 。因为recA基因比16S rRNA基因能为分类学推断提供更高的分辨率，所以保守的recA基因的引物被设计用于从嗜盐乳杆菌和Sanguibacter型菌株进行PCR扩增和测序。 9G菌株与其最近的近亲H. miurensis的recA序列同一性为99.6％，表明它是亚种。 ISLP-3菌株与其6个近亲的recA序列同一性在85.9〜90.2％之间。该结果与代表Sanguibacter属中的新物种的该菌株一致。基于此处介绍的分子研究和其他地方提供的表型特性，我们建议菌株LZ-22是一个新属和新物种的代表，拟议名称为南极雨伞属。十一月，sp。十一月菌株ISLP-3是新物种Sanguibacter gelidistatuaria sp。的代表。十一月菌株A7p-90m可能代表了拟杆菌科中的一个新家族。这些菌株的化学分类学表征正在进行中，以为拟议的分类收集更多证据。

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  • 来源
  • 会议地点 San Diego CA(US)
  • 作者

    Zhe Lyu; Elena V. Pikuta; Jacob Hagel; Genevieve R. LaBrake; Richard B. Hoover; William B. Whitman;

  • 作者单位

    University of Georgia, Department of Microbiology, Athens, 527 Biological Sciences Bldg., GA 30602-2605, USA;

    Athens State University, Department of Mathematical, Computer and Natural Sciences, 300 Beaty str., Athens, AL 35611, USA;

    Athens State University, Department of Mathematical, Computer and Natural Sciences, 300 Beaty str., Athens, AL 35611, USA;

    Athens State University, Department of Mathematical, Computer and Natural Sciences, 300 Beaty str., Athens, AL 35611, USA;

    Athens State University, Department of Mathematical, Computer and Natural Sciences, 300 Beaty str., Athens, AL 35611, USA;

    University of Georgia, Department of Microbiology, Athens, 527 Biological Sciences Bldg., GA 30602-2605, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Anaerobe; psychrophiles; glacier; PCR; Bacterial Taxonomy; Antarctica;

    机译：厌氧菌嗜冷者冰川; PCR;细菌分类学；南极洲;

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