Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay (PCR-DGGE)

机译：聚合酶链反应-变性梯度凝胶电泳法（PCR-DGGE）分析不同碳源下培养的磷酸盐积累生物

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To investigate the microbial communities of phosphate-accumulating organisms cultivated under different carbon sources, three sequencing batch reactors were operated supplied with sewage, glucose and sodium acetate as carbon sources respectively. Based on the results of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction-amplified 16S rDNA fragments, it was demonstrated that β-protebacteria and yprotebacteria only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two ones. Flavobacterium, Bacillales, Actinobacteridae and uncultured bacteria (AF527584, AF502204) that were found in a lab-scale reactor carrying out enhanced biological phosphorus removal could be detected in all three reactors.

机译：为了研究在不同碳源下培养的磷酸盐积累生物的微生物群落，分别运行了三个顺序分批反应器，分别以污水，葡萄糖和乙酸钠作为碳源。根据聚合酶链反应扩增的16S rDNA片段的变性梯度凝胶电泳（DGGE）结果，证明β-变形杆菌和γ-变形杆菌仅在1＃反应器中存在。 1＃反应器的微生物多样性超过其他两个。在所有三个反应器中都可以检测到在实验室规模的反应器中发现的黄细菌，芽孢杆菌，放线菌和未培养细菌（AF527584，AF502204）可以进行生物除磷。

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来源
《International Water Association(IWA) Conference; 20050518-20; Xi'an(CN)》|2005年|P.981-989|共9页
会议地点 Xian(CN)
作者
Yanan Liu; Shuili Yu; Guolin Jing; Bingjie Zhao; Siyuan Guo;
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作者单位

School of Municipal Environmental Engineering, Harbin Institute of Technology, Harbin, China;

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原文格式 PDF
正文语种 eng
中图分类废水的处理与利用;
关键词
enhanced biological phosphorus removal; carbon source; PCR-DGGE; phosphate-accumulating organisms; 16s rDNA;

机译：增强生物除磷碳源PCR-DGGE磷酸盐累积生物16s rDNA;

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专利

1. Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay [J] . YU Shui-li, LIU Ya-nan, JING Guo-lin Journal of Environmental Sciences . 2005,第4期

机译：聚合酶链反应-变性梯度凝胶电泳法分析不同碳源下积累的磷酸盐累积生物
2. Characterization of denitrifying phosphate-accumulating organisms cultivated under different electron acceptor conditions using polymerase chain reaction-denaturing gradient gel electrophoresis assay [J] . Johwan Ahn, Tomotaka Daidou, Satoshi Tsuneda Water Research . 2002,第2期

机译：聚合酶链反应-变性梯度凝胶电泳法表征在不同电子受体条件下培养的反硝化积累磷酸盐的生物
3. Comparison of DNA extraction methods for polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis of the infant fecal microbial communities [J] . Wenguang Fan, Xiaomin Li, Lijie Yang, African Journal of Microbiology Research . 2014,第9期

机译：婴儿粪便微生物群落的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析的DNA提取方法的比较
4. Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay (PCR-DGGE) [C] . Yanan Liu, Shuili Yu, Guolin Jing, International Water Association Conference . 2005

机译：聚合酶链反应变性梯度凝胶电泳测定不同碳源培养磷酸盐积聚生物分析（PCR-DGGE）
5. Soil community analysis along a salt gradient using denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA genes. [D] . Dutta, Partha. 2006

机译：使用PCR扩增的16S rRNA基因的变性梯度凝胶电泳，沿着盐梯度进行土壤群落分析。
6. Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling [O] . Ren-ke Wang, Xue-song He, Wei Hu, 2011

机译：使用膜结合分析结合聚合酶链反应-变性梯度凝胶电泳分析法分析口腔细菌的种间粘附
7. Influence of technological treatments on bacterial communities in tilapia (Oreochromis niloticus) as determined by 16S rDNA fingerprinting using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) [O] . Maïworé Justine, Tatsadjieu Ngoune Léopold, Goli Thierry, 2012

机译：用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）通过16S rDNA指纹图谱测定的技术处理对罗非鱼（Oreochromis niloticus）细菌群落的影响

Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay (PCR-DGGE)

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