PHOTO AUTOTROPHIC MICROPROPAGATION OF RHODODENDRON

机译：罗丹参的自养微光繁殖

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Photoautotrophic micropropagation (no sugar in the medium under a controlled environment) has many advantages over conventional photomixotrophic micropropagation (using sugar-containing medium), such as promoted growth and development of plantlets in vitro and enhanced percent survival ex vitro with many different plant species. Rhododendron is an important ornamental woody species, which often shows low multiplication rates and low percentages of rooting in vitro and survival ex vitro in conventional photomixotrophic micropropagation, and which could possibly benefit from photoautotrophic culture. In the present study, photoautotrophic culture was investigated during the multiplication and preparation stages of Rhododendron micropropagation. Based on the results, suitable conditions for obtaining a large number of Rhododendron plantlets with enhanced growth included the absence of plant growth regulators in the medium, a relatively high photosynthetic photon flux, a high COi concentration, two-leafed nodal cuttings as explants and full-strength medium. Normal roots were obtained in the absence of plant growth regulators, although the percentages of rooted plantlets were low. Further research is needed to enhance rooting in photoautotrophic micropropagation.

机译：光合自养微繁殖（在受控环境下培养基中无糖）比常规光合自养微繁殖（使用含糖培养基）具有许多优势，例如促进了幼苗的体外生长和发育以及提高了许多不同植物物种的离体存活率。杜鹃花是重要的观赏木本植物，在常规的光合营养微繁殖中，常表现出低的繁殖率和低的生根百分比，在体外和离体存活率低，并且可能受益于光合自养培养。在本研究中，在杜鹃花微繁殖的繁殖和准备阶段研究了光合自养培养。根据结果，获得大量具有增强生长能力的杜鹃花苗的合适条件包括：培养基中不存在植物生长调节剂，相对较高的光合光子通量，较高的COi浓度，作为外植体的两叶节cutting插和充分-强度中等。在没有植物生长调节剂的情况下获得了正常的根，尽管有根小植株的百分比很低。需要进一步的研究以增强光养自养微繁殖的生根。

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来源
《International Wood Biotechnology Symposium (IWBS) Mar 14-17, 2001, Narita, Chiba, Japan》|2001年|p.385-390|共6页
会议地点 Narita(JP);Narita(JP)
作者
Carmen Valero-Aracama; Sayed M. A. Zobayed; Shyamal K. Roy; Chieri Kubota; Toyoki Kozai;
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作者单位

Laboratory of Environmental Control Engineering, Faculty of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan;

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会议组织
原文格式 PDF
正文语种 eng
中图分类 Q9;
关键词
CO_2 concentration; controlled environment; explant type; IAA; IBA; medium strength; multiplication ratio; PPF; sugar; 2iP;

机译：CO_2浓度；受控环境；外植体类型IAA； IBA；中等强度倍增率PPF；糖; 2点;

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1. 动态参数控制的植物光自养微繁殖系统的架构与程序设计 [J] . 周英彪, 王奎, 肖淑惠, 农业科学与技术（英文版） . 2011,第011期
2. Commercial-scale photo-autotrophic micropropagation: applications in Irish agriculture, horticulture and forestry [J] . Roche TD, Long RD, Sayegh AJ, Acta Horticulturae . 1996,第440期

机译：商业规模的自养微繁殖技术：在爱尔兰农业，园艺和林业中的应用
3. Micropropagation of Rhododendron keiskei var. hypoglaucum Suto & Suzuki and assessment of clonal fidelity of plantlets by RAPD. [J] . Sivanesan I., Jeong ByoungRyong Propagation of Ornamental Plants . 2013,第3期

机译：杜鹃杜鹃的微繁殖。 Sug＆Suzuki的hypoglaucum以及通过RAPD评估幼苗的克隆保真度。
4. In vitro micropropagation of three rare, endangered, and endemic rhododendron species of Northeast India [J] . Mao A. A., Kaliamoorthy Seventhilingam, Ranyaphi R. A., In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association . 2011,第6期

机译：印度东北三种稀有，濒危和特有杜鹃物种的体外微繁殖
5. A preliminary experiment on photoautotrophic micropropagation of Rhododendron [C] . Carmen Valero-Aracama, Sayed M. A. Zobayed, Toyoki Kozai International Symposium on Transplant Production in Closed System for Solving the Global Issues on Environmental Conservation, Food, Resources, and Energy . 2000

机译：Rhododendron光拍微耕的初步实验
6. CLONAL PROPAGATION OF ENDANGERED NATIVE PLANTS RHODODENDRON CHAPMANII GRAY, TAXUS FLORIDANA NUTT., AND TORREYA TAXIFOLIA ARN. (MICROPROPAGATION, IN VITRO/TISSUE CULTURE, PLANT PROPAGATION, WOODY ORNAMENTALS). [D] . BARNES, LEE ROY, JR. 1985

机译：濒危原生植物杜鹃花CHAPMANII灰色，TAXUS FLORIDANA NUTT。和TORREYA TAXIFOLIA ARN的克隆繁殖。（微繁殖，体外/组织培养，植物繁殖，木质装饰物）。
7. Hydrogen Photoevolution Indicates an Increase in the Antenna Size of Photosystem I in Chlamydobotrys stellata during Transition from Autotrophic to Photoheterotrophic Nutrition [O] . Vladimir A. Boichenko, Wolfgang Wiessner, Vyacheslav V. Klimov, 1992

机译：氢光进化表明衣藻从自养营养向光养营养过渡过程中光系统I的天线大小增加。
8. Carbon Dioxide Enrichment in Autotrophic Micropropagation: Methods and Advantages [O] . Byoung Kyong Jeong, Kazuhiro Fujiwara, Toyoki Kozai 1993

机译：自营养微营养中的二氧化碳富集：方法和优点
9. Stable isotope fractionation in photosynthesis: Analysis of autotrophic competence following transformation of the chloroplast genome of Chlamydomonas. Progress report, 15 March 1989-15 June 1991. [R] . J. E. Boynton N. W. Gillham C. B. Osmond 1991

机译：光合作用中的稳定同位素分馏：分析衣藻叶绿体基因组转化后的自养能力。进展报告，1989年3月15日至1991年6月15日。

PHOTO AUTOTROPHIC MICROPROPAGATION OF RHODODENDRON

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