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首页> 外文会议>International Wood Biotechnology Symposium (IWBS) Mar 14-17, 2001, Narita, Chiba, Japan >FUNCTIONAL ANALYSIS OF PHENYLALANINEAMMONIA-LYASE GENE PROMOTER OF POPULAR
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FUNCTIONAL ANALYSIS OF PHENYLALANINEAMMONIA-LYASE GENE PROMOTER OF POPULAR

机译：流行的苯丙氨酸氨解酶基因启动子的功能分析

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摘要

Phenlyalanine ammonia-lyase (PAL) catalyzes the first step in the phenylpropanoid pathway and plays an important role in the biosynthesis of phenylpropanoid and flavonoid compounds. Cis-elements of the promoter region of g2bPAL gene, a PAL gene obtained from popular, Populus kitakamiensis, were analyzed by transient expression in carrot protoplasts and transgenic tobacco. Four conserved cis-elements, M-, G-, L- and P'-boxes in the PAL promoters of several plant species, were found in the proximal region of the g2bPAL promoter. Deletion of G-box in the promoter resulted in the loss of g2bPAL promoter activity in carrot protoplasts. In transgenic tobacco plants harboring g2bPAL-GUS, deletion of P'-box resulted in strong GUS expression in the endodermis of the stems and in the root. The findings indicate that the P'-box may play an important role in the regulation of g2bPAL expression to synthesize lignin during xylem development.
机译：苯丙氨酸氨裂解酶（PAL）催化苯丙烷途径中的第一步，并且在苯丙烷和类黄酮化合物的生物合成中起重要作用。通过在胡萝卜原生质体和转基因烟草中的瞬时表达分析了g2bPAL基因启动子区域的顺式元件，该基因是从流行的北杨（Populus kitakamiensis）获得的PAL基因。在g2bPAL启动子的近端区域中发现了几种植物物种的PAL启动子中的四个保守的顺式元件M-，G-，L-和P'-box。启动子中G-box的缺失导致胡萝卜原生质体中g2bPAL启动子活性的丧失。在带有g2bPAL-GUS的转基因烟草植物中，P'-box的缺失导致茎的内胚层和根中强烈的GUS表达。这些发现表明，在木质部发育过程中，P'-box可能在调节g2bPAL表达以合成木质素中起重要作用。

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