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The Culture Conidtions of Grifola umbellata

机译:灰树花的培养条件

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Grifola unbellata formed white and longitudinally linear hyphae, and they formed thick, peelable mycelial mat on PDA.G. unbellata also showed clamp connections on the generative hyphae, swollen hyphal tips, an hyphal sizes ranged in 0/95-2.80mivm. The mycelial growth fo G. umbellata was favorable a pH 4 and 20 degree, respectively. Under the shaking culture, G.unbellata produced mycelial masses of 15-20 mm in their diameter, and these masses showed pale yellow colors similar to those of its sclerotia in the passage of incubation time. On six tree species including log of Styrax japonica, G.umbellata formed mycelial masses of 3-5 mm in their sizes. The mycelial diameter and deisity fo G.umbellata were more favorable in the dual incubation of both Armillaria mellea nad G.umbellata than in the single incubation of G.umbellata on PDA. On the logs, there was a symbiotic ocnnection bwtween the hyphae of G.umbellata nad rhiomorphs of A.mellea in mycelial mats of G.umbellata touched closely with A.mellea; To examine ligninolytic activities of G.umbellata, lignin-degrading fungus, four strains of G.umbellata wre tested. Strain A, B, and C of G. umbellata decolorized bromophenol blue to the extent of 0.0635-0.0902, whereas stain D was negligible.
机译:Grifola unbellata形成白色且呈纵向线性的菌丝,并且在PDA上形成厚的,可剥离的菌丝垫。 unbellata还显示在生成的菌丝上有钳夹连接,菌丝尖端肿胀,菌丝大小范围为0 / 95-2.80mivm。伞形菌的菌丝体生长分别在pH 4和20度时较为有利。在摇动培养下,Unbellata菌丝体产生直径为15-20 mm的菌丝体,并且在经过一段时间后,这些菌体呈现出类似于其菌核的淡黄色。在6个树种中,包括日本菊(Styrax japonica)的原木,伞形菊(G.umbellata)形成了大小为3-5毫米的菌丝体。圆环蜜环菌和双孢菌的双重培养比在PDA上单孢子菌的双重培养更有利。在原木上,伞形线虫菌丝垫中的伞形线虫菌丝和菌丝状菌丝菌丝之间存在共生连接。为了检查伞形木霉(G.umbellata)(降解木质素的真菌)的木质素分解活性,测试了四个伞形棉(G.umbellata)菌株。伞形杆菌的菌株A,B和C使溴酚蓝脱色至0.0635-0.0902的程度,而染色剂D可忽略不计。

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