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首页> 外文会议>Life Science Systems and Applications Workshop, 2009. LiSSA 2009 >He-Ne laser enhanced cellular hydrogen peroxide production and induced modulations in metabolic activity in malignant human brain cancer: Evidence for a “by-stander” effect
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He-Ne laser enhanced cellular hydrogen peroxide production and induced modulations in metabolic activity in malignant human brain cancer: Evidence for a “by-stander” effect

机译：He-Ne激光增强了恶性人脑癌中细胞过氧化氢的产生并诱导了其代谢活性的调节：“旁观者”效应的证据

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Continuous-wave He-Ne laser exposures (Intensity=35 mW/cm2, λ=632.8nm, Fluence range: 1J/cm2 to 50 J/cm2) on non-confluent and actively dividing human malignant glioblastoma cells was found to increase the cellular production levels of H2O2. Modulations in the cellular metabolic activity were detected (through the MTS assay) three days after laser irradiation. The metabolic activity was found to be dependent on the laser dose of exposure (i.e., fluence). In addition, three days after the laser exposure, the potential laser induced “bystander” effect was tested through the transfer of growth media from laser irradiated cells onto non-irradiated cells. After two additional days of incubation (5 days post exposure), the non-laser irradiated cells were found to have a significant increase in their metabolic activities. Modulations in the metabolic activities in the non-irradiated cells were found to be fluence dependent from the initial laser exposed cells treatment conditions. The results herein support the hypothesis of an important functional role for light enhanced cellular H2O2 generation to yield bio-modulatory effects locally and at a distance. The classical “bi-phasic” modulation response of cells to light irradiation is hypothesized to depend upon the quantity of light-enhanced H2O2 molecules generated from the mitochondria and the number of cells which interact with the H2O2 molecules.
机译：连续波He-Ne激光曝光（强度= 35 mW / cm 2 ，λ= 632.8nm，通量范围：1J / cm 2 至50 J / cm 2 ）在非融合和活跃分裂的人类恶性胶质母细胞瘤细胞上被发现会增加H 2 O 2 的细胞产生水平。激光照射后三天（通过MTS分析）检测到细胞代谢活性的调节。发现代谢活性取决于暴露的激光剂量（即通量）。此外，在激光暴露三天后，通过将生长介质从激光照射的细胞转移到未照射的细胞上，测试了潜在的激光诱导的“旁观者”效应。孵育两天后（暴露后5天），发现未经激光照射的细胞的代谢活性显着增加。发现未辐照的细胞中代谢活性的调节与注视激光初始细胞处理条件的能量密度有关。本文的结果支持了光增强细胞H 2 O 2 产生以在局部和远距离产生生物调节作用的重要功能作用的假设。假设细胞对光照射的经典“双相”调制响应取决于线粒体和线粒体产生的光增强的H 2 O 2 分子的数量。与H 2 O 2 分子相互作用的细胞数。

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