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Detection of multiple overlapping bands of known amplitude, with application to DNA fingerprinting

机译：检测已知幅度的多个重叠谱带，并应用于DNA指纹图谱

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One of the technologies in DNA mapping is the preparation of electrophoretic gels, which when stained and imaged electronically contain a number of diffuse rectangular objects, called "bands", several pixels in area, which must be detected and localized. This paper describes an algorithm which is central to the automated analysis of such gels, part of a large software product now in use for mapping projects at several genomics laboratories. The problem addressed is that of detecting multiple overlapping pulses of known shape and amplitude. The basic algorithm is one of analysis-by-synthesis, where pulses are added one by one to a forward synthesis model, with revisit to all pulses after each new pulse is added. Because the amplitudes are known there is no risk of significantly overfitting the data with additional model terms. The cost function utilized is a weighted least-squares function, which gives more weight to the shape of the test signal and less to its overall amplitude. Application of this algorithm in the larger context of DNA fingerprinting is discussed.
机译：DNA作图的技术之一是制备电泳凝胶，当对其进行染色和电子成像时，它包含许多被称为“条带”的扩散矩形物体，其面积为几个像素，必须对其进行检测和定位。本文介绍了一种算法，对于这种凝胶的自动分析至关重要，该算法是大型软件产品的一部分，目前已在多个基因组学实验室用于映射项目。解决的问题是检测形状和振幅已知的多个重叠脉冲。基本算法是逐个分析的一种，其中将脉冲逐个添加到正向合成模型，并在添加每个新脉冲后重新访问所有脉冲。由于振幅是已知的，因此没有使用额外的模型项显着过度拟合数据的风险。所使用的代价函数是加权最小二乘函数，该函数对测试信号的形状具有更大的权重，而对它的整体幅度却具有较小的权重。讨论了该算法在更大范围的DNA指纹识别中的应用。

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