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Raman versus CARS microscopy: when one is better than the other

机译:拉曼与CARS显微镜:何时一个比另一个更好

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Since the first introduction of Raman microscope in 1973, optical and laser technology has made a tremendous step forward. However, despite of the obvious advantages of being very informative and nondestructive method of studying biological samples, spontaneous Raman scattering suffers from a series of limitations, such as a fluorescent background and a low signal level. Nonlinear Raman spectroscopy, and, in particular, spectroscopy of coherent anti-Stokes Raman scattering (CARS) can resolve most of the problems associated with conventional Raman spectroscopy. In this report, the most critical issues of the CARS microspectroscopy setup design are reviewed, and several exciting potential applications of the broadband CARS microspectroscopy, where the CARS microscopy has an advantage with respect to Raman microscopy, are outlined.
机译:自1973年拉曼显微镜问世以来,光学和激光技术已迈出了一大步。然而,尽管作为研究生物样品的信息非常丰富且非破坏性的方法具有明显的优势,但自发拉曼散射仍然受到一系列限制,例如荧光背景和低信号水平。非线性拉曼光谱,尤其是相干反斯托克斯拉曼散射(CARS)的光谱,可以解决与传统拉曼光谱相关的大多数问题。在本报告中,回顾了CARS显微镜设置设计中最关键的问题,并概述了宽带CARS显微镜的一些令人兴奋的潜在应用,其中CARS显微镜相对于拉曼显微镜具有优势。

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