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Bioconversion of Fumaric Acid to Succinic Acid by Recombinant E. coll

机译：重组大肠杆菌将富马酸生物转化为琥珀酸

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Succinic acid was produced efficiently from fumaric acid by a recom-binant E. coli strain DH5α/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. L-Malic acid became the major fermentation product under these conditions. Provision of glucose (5-30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.
机译：含有多拷贝富马酸酯还原酶基因的重组大肠杆菌菌株DH5α/ pGC1002可从富马酸高效生产琥珀酸。研究了初始富马酸和葡萄糖浓度对琥珀酸生产的影响。从50至64 g / L富马酸开始，琥珀酸在48.5小时内达到41至60 g / L以上。在初始富马酸浓度为90 g / L时观察到了显着的底物抑制作用。在这些条件下，L-苹果酸成为主要的发酵产物。向发酵培养基中提供葡萄糖（5-30 g / L）可将初始琥珀酸的生产速率提高两倍。

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