• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>European Brewery Convention Congress >PCR-DHPLC (Polymerase Chain Reaction - Denaturing High Performance Liquid Chromatography): a potential novel method for rapid screening of mixed yeast populations
【24h】

PCR-DHPLC (Polymerase Chain Reaction - Denaturing High Performance Liquid Chromatography): a potential novel method for rapid screening of mixed yeast populations

机译：PCR-DHPLC（聚合酶链反应-变性高效液相色谱法）：一种快速筛选混合酵母种群的潜在新方法

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The rapid detection, identification and separation of spoilage and brewing yeasts are of serious interest for the quality control of the beer production process. The potential of the PCR-DHPLC (Polymerase Chain Reaction - Denaturing High Performance Liquid Chromatography) method was studied to detect the presence of spoilage yeast populations in single and mixed cultures. PCR was performed with universal primers amplifying the ITS 1 and ITS2 rRNA genes of yeast species within a mixed population, followed by DHPLC to separate the different PCR-products. Applying this method, 21 of the 25 yeast species tested were clearly separated from each other, showing the potential of PCR-DHPLC in the quality control process. The other four yeast species formed two clusters. Additionally some Saccharomyces cerevisiae non brewing strains and strains very closely related to brewing yeast strains could be differentiated from brewing yeast strains. Using a Primer pair specific to S. cerevisiae yeasts the PCR- DHPLC method showed the potential to differentiate pure brewing culture yeast strains.
机译：对于腐败和酿造酵母的快速检测，鉴定和分离对于啤酒生产过程的质量控制非常重要。研究了PCR-DHPLC（聚合酶链反应-变性高效液相色谱）方法的潜力，以检测单一和混合培养物中腐败的酵母菌群的存在。用通用引物进行PCR，以扩增混合种群中酵母菌种的ITS 1和ITS2 rRNA基因，然后进行DHPLC分离不同的PCR产物。应用该方法，可以清楚地将测试的25种酵母中的21种彼此分离，显示出PCR-DHPLC在质量控制过程中的潜力。其他四种酵母菌形成了两个簇。另外，可以将某些酿酒酵母非酿造菌株和与酿造酵母菌株密切相关的菌株与酿造酵母菌株区分开。使用特异于酿酒酵母酵母的引物对，PCR-DHPLC方法显示了区分纯酿造培养酵母菌株的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号