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首页> 外文会议>European Brewery Convention Congress >PCR-DHPLC (Polymerase Chain Reaction - Denaturing High Performance Liquid Chromatography): a potential novel method for rapid screening of mixed yeast populations
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PCR-DHPLC (Polymerase Chain Reaction - Denaturing High Performance Liquid Chromatography): a potential novel method for rapid screening of mixed yeast populations

机译：PCR-DHPLC（聚合酶链反应 - 变性高效液相色谱）：一种潜在的快速筛选混合酵母种群的新方法

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摘要

The rapid detection, identification and separation of spoilage and brewing yeasts are of serious interest for the quality control of the beer production process. The potential of the PCR-DHPLC (Polymerase Chain Reaction -^sDenaturing High Performance Liquid Chromatography) method was studied to detect the presence of spoilage yeast populations in single and mixed cultures. PCR was performed with universal primers amplifying the ITS 1 and ITS2 rRNA genes of yeast species within a mixed population, followed by DHPLC to separate the different PCR-products. Applying this method, 21 of the 25 yeast species tested were clearly separated from each other, showing the potential of PCR-DHPLC in the quality control process. The other four yeast species formed two clusters. Additionally some Saccharomyces cerevisiae non brewing strains and strains very closely related to brewing yeast strains could be differentiated from brewing yeast strains. Using a Primer pair specific to S. cerevisiae yeasts the PCR-^sDHPLC method showed the potential to differentiate pure brewing culture yeast strains.
机译：腐败和酿造酵母的快速检测，鉴定和分离对啤酒生产过程的质量控制具有严重兴趣。研究了PCR-DHPLC（聚合酶链反应 - ^ SENATULES高效液相色谱）方法的电位，以检测单一和混合培养物中腐败酵母群的存在。通过扩增其在混合群体内扩增其1和ITS2酵母物种RRNA基因的通用引物进行PCR，然后用DHPLC分离不同的PCR产物。施加该方法，21种测试的21种测试的21种彼此清楚地分离，显示PCR-DHPLC在质量控制过程中的潜力。另外四种酵母种形成了两种簇。另外，一些酿酒酵母与酿造酵母菌株非常密切地密切相关的酿酒酵母酿造菌株和菌株可由酿造酵母菌菌株分化。使用特异于S.酿酒酵母酵母的引物对PCR-^ SDHPLC方法显示出纯酿造培养酵母菌株的可能性。

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