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Identification of one polysaccharide gene and analysis of differentially expressed proteins from bioflocculant producing strain of F2

机译:鉴定一种多糖基因并分析F2生物絮凝剂产生菌株中差异表达的蛋白质

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F2 is one of two compound bioflocculant producing strains with good genetic stability and high flocculating activity.The core sequence of exoL was amplified from the F2 genome by the method of polymerase chain reaction,identifying that the effective polysaccharide component of bioflocculant produced by F2 was succinoglycan.The changes of protein complexes of F2 grown in minimal media versus bioflocculant media were researched by SDS-PAGE,indicating that in bioflocculant media compared with minimal media,the relative content of two protein complexes were obviously increased and the relative content of one protein complexes was obviously decreased in gels.It was three differentially expressed protein complexes that can probably induce the production of large amount of the effective polysaccharide component of bioflocculant produced by F2.The research can provide a step to reveal the molecular flocculating mechanisms of compound bioflocculant producing strains.
机译:F2是两个具有良好遗传稳定性和絮凝活性的复合生物絮凝剂生产菌株之一。采用聚合酶链反应的方法从F2基因组中扩增出exoL的核心序列,确定F2产生的生物絮凝剂的有效多糖成分为琥珀聚糖。通过SDS-PAGE研究了在基本培养基和生物絮凝剂培养基中生长的F2蛋白复合物的变化,表明与基本培养基相比,在生物絮凝剂培养基中,两种蛋白质复合物的相对含量明显增加,而一种蛋白质复合物的相对含量明显增加。在凝胶中明显减少了。这是三种差异表达的蛋白质复合物,可能诱导了F2产生的大量生物絮凝剂有效多糖成分的产生。该研究可为揭示复合生物絮凝剂产生菌株的分子絮凝机理提供一个步骤。 。

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