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首页> 外文期刊>Indian Journal of Microbiology >Identification of Genes Differentially Expressed Between Ochratoxin-Producing and Non-Producing Strains of Aspergillus westerdijkiae
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Identification of Genes Differentially Expressed Between Ochratoxin-Producing and Non-Producing Strains of Aspergillus westerdijkiae

机译：西洋曲霉产Stra曲霉菌株和非产ch曲霉菌株之间差异表达基因的鉴定

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摘要

Approximately 70 % of Aspergillus westerdijkiae strains are able to produce ochratoxin A (OTA), a nephrotoxic and carcinogenic mycotoxin which have been found in cereal and food commodities. Despite of its importancethereis,uptonow,noinformationavailableaboutwhich genes are differentially expressed between A. westerdijkiae ochratoxin-producing and non-producing strains. Using cDNA RDA approach we successfully sequenced 231 raw ESTs expected to be enriched in the ochratoxin-producing strain.BLASTXsearchesagainstthepublicdatabasesshowed that of these, 205 ESTs (79 %) exhibited significant similarities with proteins of known functions, 28 ESTs (11 %) had matches to hypothetical proteins, and the remaining 27 ESTs(10 %) had no significant hits. EST alignment resulted in a total of 14 non-redundant consensus sequences. Three putative genes encoding oxidoreductases were validated as upexpressed in the OTA producer strain using RT-qPCR approach. The expression of the putative genes encoding a cytochrome P450 family protein, 3-hydroxyphenylacetate-6-hydroxylase, and endoplasmic reticulum oxidoreductin were higher (32-, 2.8- and 20-fold respectively) in the OTA producer strain compared to the non-producer strain.
机译：大约70％的西曲霉菌株能够产生曲霉毒素A（OTA），曲霉毒素A是一种在谷物和食品中发现的具有肾毒性和致癌性的霉菌毒素。尽管它的重要性，但尚无信息可证明在产A. westerdijkiae ra曲霉毒素的菌株和不产A. westerdijkiae ochratoxin的菌株之间差异表达的基因。使用cDNA RDA方法，我们成功地测序了231种预期在产曲毒素的菌株中富集的原始EST.BLASTX对公共数据库的研究表明，其中205个EST（79％）与已知功能的蛋白质表现出显着相似性，其中28个EST（11％）与已知功能的蛋白质具有相似性假设的蛋白质，其余27个EST（10％）没有明显的打击。 EST比对产生总共14个非冗余共有序列。使用RT-qPCR方法验证了三个假定的编码氧化还原酶的基因在OTA生产菌株中的高表达。与非生产者相比，OTA生产者菌株中编码细胞色素P450家族蛋白，3-羟苯基乙酸-6-羟化酶和内质网氧化还原蛋白的推定基因的表达更高（分别为32倍，2.8倍和20倍）。应变。

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