Sequence length variation of internal genic space of 16S rDNA-23S rDNA in bacterium with high yield of hydrogen production

机译：高产氢细菌中16S rDNA-23S rDNA内部基因空间的序列长度变异

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To develop the identification of species for fermentative biohydrogen-producing bacterium, scholars have found a method which is based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions. In the study, a large fragment of the rDNA operon, including the 16S rDNA, the intergenic spacer region (ISR) and approximately 2000 bases of the 23S rDNA, were polymerasechain reaction (PCR) amplified. The PCR amplification of the genomic DNA of Leptonema ilk strain 3055 using primers directed against conserved regions of the rRNA operon provided evidence that the 16S and 23S rRNA genes were linked via an intergenic spacer region. The sequencing of the intergenic spacer region indicated that it was 435 nucleotides in length and sequence similarity searches revealed that it bore no homology to any known sequences including tRNA available in databases.

机译：为了开发用于鉴定发酵性生物产氢细菌的物种，学者们发现了一种基于PCR扩增16S rRNA基因（rDNA）-23S rDNA基因间区域的方法。在这项研究中，rDNA操纵子的大片段，包括16S rDNA，基因间隔区（ISR）和23S rDNA的大约2000个碱基，被聚合酶链反应（PCR）扩增了。使用针对rRNA操纵子保守区域的引物对Leptonema ilk菌株3055的基因组DNA进行PCR扩增，提供了16S和23S rRNA基因通过基因间隔区连接的证据。基因间隔区的测序表明，它的长度为435个核苷酸，序列相似性搜索显示，它与数据库中已知的任何已知序列（包括tRNA）没有同源性。

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《International conference on environmental biotechnology and materials engineering;EBME 2011》|2011年|p.1413-1416|共4页
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Yongfeng Li; Yixuan Wang; Lu Wang; Zhanqing Wang;
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中图分类环境生物学;环境生物学;
关键词
Biohydrogen; H2-producing bacterium; 16S-23S rDNA intergenic region; Length variation;

机译：生物氢;产H2细菌; 16S-23S rDNA基因间区;长度变异;

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1. Variation of 16S-23S internally transcribed spacer sequence and intervening sequence in rDNA among the three major Agrobacterium species [J] . Bautista-Zapanta Jn, Arafat HH, Tanaka K, Microbiological Research . 2009,第6期

机译：三个主要农杆菌属物种中rSDNA中16S-23S内转录间隔区序列和插入序列的变异
2. Genetic variation in 16S-23S rDNA internal transcribed spacer regions and the possible use of this genetic variation for molecular diagnosis of Bacteroides species. [J] . Kuwahara T, Norimatsu I, Nakayama H, Microbiology and Immunology . 2001,第3期

机译：16S-23S rDNA内部转录间隔区的遗传变异以及该遗传变异在拟杆菌属分子诊断中的应用。
3. Assessment of genetic diversity, and phylogenetic relationships based on ribosomal DNA repeat unit length variation and Internal Transcribed Spacer (ITS) sequences in chickpea (Cicer arietinum) cultivars and its wild species [J] . Apekshita Singh, R. M. Devarumath, S. RamaRao, Genetic Resources and Crop Evolution . 2008,第1期

机译：基于核糖体DNA重复单位长度变异和内部转录间隔区（ITS）序列的鹰嘴豆（Cicer arietinum）品种及其野生物种的遗传多样性和系统发生关系的评估
4. Sequence length variation of internal genic space of 16S rDNA-23S rDNA in bacterium with high yield of hydrogen production [C] . Yongfeng Li, Yixuan Wang, Lu Wang, International Conference on Environmental Biotechnology and Materials Engineering . 2011

机译：具有高产氢气产量的氢气中16S rDNA-23s rDNA的内部基因空间的序列长度变化
5. Identification and phylogenetic analysis of southeastern United States earthworm species using 16S rDNA and CO1 sequences. [D] . Leontieva, Yulia A. 2007

机译：使用16S rDNA和CO1序列鉴定和鉴定美国东南worm物种。
6. Genotypic Characterization of Bradyrhizobium Strains Nodulating Endemic Woody Legumes of the Canary Islands by PCR-Restriction Fragment Length Polymorphism Analysis of Genes Encoding 16S rRNA (16S rDNA) and 16S-23S rDNA Intergenic Spacers Repetitive Extragenic Palindromic PCR Genomic Fingerprinting and Partial 16S rDNA Sequencing [O] . Pablo Vinuesa, Jan L. W. Rademaker, Frans J. de Bruijn, 1998

机译：通过编码16S rRNA（16S rDNA）和16S-23S rDNA基因间隔子重复的外基因回文PCR基因组指纹图谱和部分16S rDNA Sequence的PCR-限制性片段长度多态性分析对加那利群岛特有木本植物结瘤的根瘤菌菌株进行基因型鉴定。
7. Genetic analyses of the genus Nocardioides and related taxa based on 16S-23S rDNA internally transcribed spacer sequences [O] . J.-H. Yoon, S. T. Lee, Y.-H. Park 1998

机译：基于16S-23S RDNA内部转录间隔序列的Nocardioides和相关分类群的遗传分析

Sequence length variation of internal genic space of 16S rDNA-23S rDNA in bacterium with high yield of hydrogen production

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