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首页> 外文会议>ICoMST 2011;International conference of meat science and technology >Different methods to identify yeast population associated with dry-cured lacon, a traditional meat product from North-West of Spain
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Different methods to identify yeast population associated with dry-cured lacon, a traditional meat product from North-West of Spain

机译：不同的方法来鉴定与干腌腊肉有关的酵母种群，干腊肉是西班牙西北部的传统肉类产品

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In the present study, three different methods were used to identify yeast isolated from dry-cured lacon, a traditional meat product from Galicia (North-West of Spain). A total of 145 strains were taken randomly from drying-ripening stage (from 7 to 84 days) and identified using the API 20C AUX kit (BioMerieux-Vitek, Hazelwood, Mo), restriction fragment length polymorphism (RFLP) analysis of 5.8S ribosomal RNA gene and two internal transcribed spacers (ITS1 and ITS2), and the last one, sequencing the same region restriction. Six different yeast species were identified representing five genera: Debaryomyces, Candida, Cryptococcus, Rhodotorula and Rhodosporium. Among the isolated lacon samples, Debaryomyces hansenii was the predominant specie (77,9%). Sequencing of the 5.8S-ITS1 region yielded the best results in terms of correct identification. The commercial identification kit API 20C AUX was able to correctly identify only 55,2% of the isolates.
机译：在本研究中，使用了三种不同的方法来鉴定从干腌的熏肉中分离出的酵母，干熏的熏肉是来自加利西亚（西班牙西北部）的传统肉制品。从干燥成熟阶段（7天至84天）随机抽取了145株菌株，并使用API​​ 20C AUX试剂盒（BioMerieux-Vitek，Hazelwood，Mo）进行了鉴定，对5.8S核糖体进行了限制性片段长度多态性（RFLP）分析。 RNA基因和两个内部转录间隔子（ITS1和ITS2），最后一个，对相同区域的限制进行测序。确定了代表五个属的六个不同的酵母种类：拟杆菌属，假丝酵母​​，隐球菌，杜鹃花属和杜鹃花属。在分离出的熏衣草样品中，汉逊德巴利酵母是最主要的种类（77.9％）。就正确识别而言，对5.8S-ITS1区进行测序可获得最佳结果。商业鉴定试剂盒API 20C AUX只能正确鉴定55.2％的分离株。

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