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首页> 外文会议>International Symposium on Micro-NanoMechatronics and Human Science >Complex Formations between Artificial RNA-DNA Chimera Nucleic Acids and RNA Modification Enzyme
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Complex Formations between Artificial RNA-DNA Chimera Nucleic Acids and RNA Modification Enzyme

机译：人工RNA-DNA嵌合核酸和RNA改性酶之间的复杂形成

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In living cells, RNA modification enzymes form multiple styles of RNA-protein complexes with various precursor RNA species for generation of mature RNA molecules. In general, however, preparation of the processing-modification complex is very difficult, because the complex is dissociated according to progress of the enzymatic reactions. Until now, several ways to prevent the dissociation of complexes have been devised, for examples utilization of small substrate analogues and/or disruption of the catalytic center of the enzyme. However, in the case of some RNA methyltransferases such as tRNA (Gm18) methyltransferase [TrmH], these approaches are not successful. TrmH enhances the affinity for RNA only in the presence of the methyl group donor, S-adenosyl-L-methionine (AdoMet). AdoMet analogues do not work like AdoMet in the case of TrmH, because TrmH requires the activated methyl group in AdoMet for structural change of the enzyme. Furthermore, disruptions of the catalytic center (Arg41 residue) by amino acid substitutions cause perturbation of the local protein structure around the catalytic center and result in decrease of the affinity for RNA. Therefore, a new approach for stabilization of the RNA-protein complex is desired. In the current study, we prepared artificial RNA-DNA chimera nucleic acids, in which ribonucleotide(s) is substituted by deoxyribonucleotide(s). Some of the artificial nucleic acids formed stable complexes with TrmH.
机译：在活细胞中，RNA改性酶形成多种风格的RNA蛋白复合物，其具有各种前体RNA物种用于产生成熟RNA分子。然而，通常，加工改性复合物的制备非常困难，因为根据酶促反应的进展，复合物是解离的。到目前为止，已经设计了几种防止复合物解离的方法，用于实施小衬底类似物和/或酶催化中心的破坏。然而，在一些RNA甲基转移酶如TRNA（GM18）甲基转移酶[TRMH]的情况下，这些方法不成功。 TRMH仅在甲基供体，S-腺苷-1-甲硫氨酸（Adomet）存在下对RNA的亲和力。在TRMH的情况下，ADOST类似物不像Adomet一样工作，因为TRMH需要Adomet中的活化甲基用于酶的结构变化。此外，氨基酸取代的催化中心（Arg41残基）的破坏引起催化中心周围的局部蛋白质结构的扰动，并导致RNA的亲和力降低。因此，需要一种稳定RNA蛋白质复合物的新方法。在目前的研究中，我们制备人工RNA-DNA嵌合核酸，其中核糖核苷酸被脱氧核苷酸代替。一些人工核酸用TRMH形成稳定的配合物。

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