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Comparative Researches on Two Kinds of Staining Method of Acetylcholinesterase Isozymes

机译:两种乙酰胆碱酯酶同工酶染色方法的比较研究

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The staining effects of two staining methods of acetylcholinesterase isozymes were compared.One using acetylthiocholine iodide,K3[Fe(CN)6] and CuSO4 was named as AFCu method,and the other one using acetylthiocholine iodide,K3[Fe(CN)6] and FeCl3 was named as AFFe method.The staining effect of former was better.By physiological researches the acetylcholinesterase (AChE,EC.3.1.1.7),xisting in red cells,liver,spleen,nerve and muscle,plays an important role in erveconduction processes in lives[1].For its close relationships with Alzheimer's disease (AD),close attention to the function of the AChE in the formation and development of lzheimer's disease[2] and the screen of its inhibitors [1,3,4]have been paid in the academic world.Otherwise,to meet the need of rapid detection of organophosphorus pesticides and monitoring of environmental pollution [1,5],the AChE has also become more and more important.Two staining methods of AChE isozymes will be compared,in order to be helpful to the researches on the AChE.
机译:比较了两种乙酰胆碱酯酶同工酶的染色效果。一种使用碘化乙酰硫胆碱K3 [Fe(CN)6]和CuSO4的方法称为AFCu法,另一种使用碘化乙酰胆碱酯化K3 [Fe(CN)6]。前者的染色效果更好。通过生理学研究乙酰胆碱酯酶(AChE,EC.3.1.1.7),存在于红细胞,肝脏,脾脏,神经和肌肉中,在神经传导中起重要作用。生命过程[1]。由于它与阿尔茨海默氏病(AD)密切相关,因此应密切注意AChE在阿尔茨海默氏病形成和发展中的功能[2]及其抑制剂的筛选[1,3,4]否则,为了满足快速检测有机磷农药和监测环境污染的需要[1,5],AChE也变得越来越重要。将比较两种AChE同工酶染色方法,以帮助研究他在AChE上。

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