• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>IEEE International Conference on Nanotechnology >Enzyme Conjugation and Biosensing with Quantum Dots: a Photoluminesceece Study
【24h】

Enzyme Conjugation and Biosensing with Quantum Dots: a Photoluminesceece Study

机译：用量子点酶缀合和生物溶解：光荧光研究

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper we investigate the enzyme conjugation of CdSe/ZnS core/shell quantum dots (QDs) by means of their photoluminescent properties, as well as the influence of the enzymatic reaction on these properties. The QDs were first transferred from toluene to water by encapsulation in poly(ethylene glycol)-phospholipid micelles. The water solubilized QDs were bioconjugated with the enzymes glucose oxidase (GOX) and horseradish peroxidase (HRP). For covalent coupling the crosslinkers l-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and hydroxysulfo-succinimide (sulfo-NHS) were used. Photoluminescence (PL) measurements showed that EDC had a large effect on the PL intensity. Also, the PL intensity increased in PBS buffer as compared to water. The enzyme conjugation caused a noticeable shift in the PL peak wavelength, but had a minor effect on the PL intensity. Sensing experiments confirmed the well-known quenching of PL by H_2O_2- Similarly, the PL intensity was sensitive to glucose in the m.M range in the presence of GOX. This can be understood since the conversion of glucose by GOX yields H_2O_2 as a by-product. Measurements of the quantum yield (QY) showed increased values for QD solutions treated with EDC. The QY decreased with increasing glucose concentration in the presence of GOX. The change in QY was accompanied by a change in pH, suggesting a correlation between pH and QY.
机译：在本文中，我们通过它们的光致发光性能研究CDSE / ZnS核心/壳量子点（QDS）的酶缀合，以及酶促反应对这些性质的影响。首先通过封装聚（乙二醇）-phophipid胶束将QD从甲苯转移到水中。将溶解的QDS与酶葡萄糖氧化酶（GOX）和辣根过氧化物酶（HRP）进行生物缀合。对于共价偶联，使用交联剂L-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）和羟基硫酰琥珀酰亚胺（磺酰胺）。光致发光（PL）测量表明，EDC对PL强度有很大影响。此外，与水相比，PBS强度在PBS缓冲液中增加。酶缀合在PL峰值波长中引起明显的偏移，但对PL强度有微小的影响。感测实验证实了H_2O_2的众所周知的PL猝灭，类似地，PL强度在GOX存在下对M.M范围内的葡萄糖敏感。这可以理解，因为GOX的葡萄糖转化为副产物，因此葡萄糖产生H_2O_2。量子产率（QY）的测量显示了用EDC处理的QD溶液的增加的值。随着GOX的存在，QY随着葡萄糖浓度的增加而降低。 QY的变化伴随着pH的变化，表明ph和qy之间的相关性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号