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首页> 外文会议>International conference on applied biotechnology >Metabolic Analysis of a Corynebacterium glutamicum IdhA Mutant During an Efficient Succinate Production Using pH-Control Under Oxygen Deprivation
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Metabolic Analysis of a Corynebacterium glutamicum IdhA Mutant During an Efficient Succinate Production Using pH-Control Under Oxygen Deprivation

机译：谷氨酸棒杆菌IdhA突变体在缺氧条件下使用pH控制进行高效琥珀酸生产的代谢分析

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Corynebacterium glutamicum produce succinate and lactate from glucose under oxygen deprivation without growth. By deleting the lactate dehydro-genase genes (ldhA), lactate formation could be completely abolished and yield of succinic acid increased up to 0.78 g g~(-1). With increasing concentration of carbonate, which could regular the pH of culture, the glucose consumption rate has been improved, leading to increase the succinic acid production rates. Interestingly, depending on the pH conditions, there is a sharp increase in the presence of higher concentration carbonate. This phenomenon was investigated by the key metabolic enzymes analysis during different pH condition. A pyruvate carboxylase and malate dehydrogenise played a significant role in succinic production under oxygen deprivation. Succinic acid production by a ldhA mutant was investigated with free pH and constant pH 8.0. Succinic acid production and yield were higher under constant pH 8.0 conditions compared with culture with free pH. In an efficient succinic acid production process, C. glutamicum ATCC 13032 ΔldhA accumulated 67.2 g l~(-1) succinic acid within 48 h with an average succinic production rate 0.14 g l~(-1) per cells (dry weight) (cdw) per hour.
机译：谷氨酸棒杆菌在缺氧的情况下从葡萄糖中产生琥珀酸和乳酸而无生长。通过删除乳酸脱氢酶基因（ldhA），可以完全消除乳酸的形成，琥珀酸的产量增加到0.78 g g〜（-1）。随着碳酸盐浓度的增加（可以调节培养液的pH值），葡萄糖的消耗速率得到了改善，从而提高了琥珀酸的生产率。有趣的是，取决于pH条件，在较高浓度的碳酸盐的存在下急剧增加。通过在不同pH条件下的关键代谢酶分析研究了这种现象。丙酮酸羧化酶和苹果酸脱氢在缺氧条件下的琥珀酸生产中起重要作用。在自由pH和恒定pH 8.0下研究了ldhA突变体产生的琥珀酸。在恒定pH 8.0条件下，琥珀酸的产量和收率要高于在自由pH条件下培养的琥珀酸。在高效的琥珀酸生产过程中，谷氨酸棒杆菌ATCC 13032ΔldhA在48小时内积累了67.2 gl〜（-1）琥珀酸，平均每细胞（干重）（cdw）的琥珀酸生产率为0.14 gl〜（-1）小时。

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