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首页> 外文会议>International Workshop on Magnetic Particle Imaging >Biomaterials for Regenerative Medicine: Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Stem Cells
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Biomaterials for Regenerative Medicine: Cytotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Stem Cells

机译：再生药物的生物材料：干细胞中超顺磁性氧化铁纳米颗粒的细胞毒性

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Detrimental effects of nanoparticles on cell viability, cell growth and morphology have been an ongoing topic in the field of nanoparticle tissue engineering, cell labeling, and drug delivery. Establishing biocompatible nanoparticles is particularly important for stem cell -based therapies and cell-tracking by magnetic particle imaging in regenerative medicine. Recently, magnetic particle imaging (MPI) has been presented as a new method for the measurement of the spatial distribution of superparamagnetic iron oxide nanoparticles (SPIOs). Spatial resolution and signal to noise ratio of MPI depend on the particle quality. Here we developed dextran-coated SPIOs for magnetic particle imaging and analyzed their stability and hydrodynamic diameter by photon cross correlation spectroscopy (PCCS). The uptake of SPIOs and the morphology of labeled human adult stem cells were examined by confocal laser scanning microscopy. Labeled stem cell growth was monitored by the xCELLigence system. We focused on commonly used in vitro assays for estimation of cell viability and cell death. The newly developed SPIOs revealed a low signal to noise ratio. Dextrancoated SPIOs had no significant influence on cell growth and viability of human adult stem cells. Our data support dextran-coated magnetic nano-particles as a well-tolerated and promising tool for further surface modifications and stem cell -based therapies.
机译：纳米颗粒对细胞活力，细胞生长和形态的不利影响是纳米粒子组织工程，细胞标记和药物递送领域的持续话题。建立生物相容性纳米颗粒对干细胞基于干细胞的疗法和细胞跟踪尤其重要，通过再生医学中的磁性颗粒成像。最近，磁性颗粒成像（MPI）已经呈现为测量超顺磁性氧化铁纳米颗粒（SpiOS）的空间分布的新方法。 MPI的空间分辨率和信噪比取决于粒子质量。在这里，我们开发了用于磁性颗粒成像的葡聚糖涂层孢子，并通过光子交叉相关光谱（PCCs）分析它们的稳定性和流体动力学直径。通过共聚焦激光扫描显微镜检查分子的摄取和标记的人成人干细胞的形态。通过Xcelligence系统监测标记的干细胞生长。我们专注于常用于体外测定以估计细胞活力和细胞死亡。新开发的斯普里斯揭示了低信噪比。 Dextrancoated Spios对人类成人干细胞的细胞生长和活力没有显着影响。我们的数据支持葡聚糖涂覆的磁性纳米颗粒作为良好的容忍和有前途的工具，用于进一步的表面修饰和干细胞的疗法。

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