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首页> 外文期刊>Journal of applied toxicology >In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
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In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests

机译：超顺磁性氧化铁纳米粒子对神经元和神经胶质细胞的体外细胞毒性。通过可行性测试评估纳米粒子的干扰

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Superparamagnetic iron oxide nanoparticles (ION) have attracted great interest for use in several biomedical fields. In general, they are considered biocompatible, but little is known of their effects on the human nervous system. The main objective of this work was to evaluate the cytotoxicity of two ION (magnetite), coated with silica and oleic acid, previously determining the possible interference of the ION with themethodological procedures to assure the reliability of the results obtained. Human neuroblastoma SHSY5Y and glioblastoma A172 cells were exposed to different concentrations of ION (5-300 mu g ml(-1)), prepared in complete and serum-free cell culture medium for three exposure times (3, 6 and 24 h). Cytotoxicity was evaluated by means of the MTT, neutral red uptake and alamar blue assays. Characterization of the main physical-chemical properties of the ION tested was also performed. Results demonstrated that both ION could significantly alter absorbance readings. To reduce these interferences, protocols were modified by introducing additional washing steps and cell-free systems. Significant decreases in cell viability were observed for both cell lines in specific conditions by all assays. In general, oleic acid-coated ION were less cytotoxic than silica-coated ION; besides, a serum-protective effect was observed for both ION studied and cell lines. These results contribute to increase the knowledge of the potential harmful effects of ION on the human nervous system. Understanding these effects is essential to establish satisfactory regulatory policies on the safe use of magnetite nanoparticles in biomedical applications. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：超顺磁性氧化铁纳米颗粒（ION）在许多生物医学领域中引起了极大的兴趣。通常，它们被认为具有生物相容性，但对它们对人类神经系统的影响知之甚少。这项工作的主要目的是评估涂覆有二氧化硅和油酸的两种ION（磁铁矿）的细胞毒性，事先确定ION对方法学程序的可能干扰，以确保所得结果的可靠性。将人类神经母细胞瘤SHSY5Y和胶质母细胞瘤A172细胞暴露于不同浓度的ION（5-300μg ml（-1））中，该离子在完全无血清的细胞培养基中制备三个暴露时间（3、6和24 h）。通过MTT，中性红摄取和阿拉玛蓝测定法评估细胞毒性。还对测试的离子的主要物理化学性质进行了表征。结果表明，两种离子均可显着改变吸光度读数。为了减少这些干扰，通过引入其他洗涤步骤和无细胞系统对方案进行了修改。通过所有测定，在特定条件下两种细胞系均观察到细胞活力的显着降低。通常，油酸包覆的ION的细胞毒性比二氧化硅包覆的ION低。此外，对所研究的ION和细胞系均观察到了血清保护作用。这些结果有助于增加对离子对人类神经系统潜在有害作用的了解。对于在生物医学应用中安全使用磁铁矿纳米颗粒建立令人满意的监管政策，了解这些影响至关重要。版权所有（C）2015 John Wiley＆Sons，Ltd.

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